Gas chromatography-mass spectrometry (GC-MS) techniques for metabolic flux analysis of the Bifido shunt pathway

被引：3
作者：
Price, Neil P.
Whitehead, Terence R.
Cote, Gregory L.
机构：
[1] USDA ARS, Natl Ctr Agr Utilizat Res, Bioprod & Biocatalysis Res Unit, Peoria, IL 61604 USA
[2] USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biotechnol Res Unit, Peoria, IL 61604 USA
来源：
BIOCATALYSIS AND BIOTRANSFORMATION | 2006年 / 24卷 / 1-2期
关键词：
bifidobacterium; bifido shunt; flux; mass spectrometry; metabolism;
D O I：
10.1080/10242420500533325
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The presence of the Bifido shunt in Bifidobacterium is predicted to lead to the uptake and metabolic conversion of fructose to acetate and lactate. We propose an approach to quantifying the carbon flux through the Bifido shunt by measuring specific C-13-labeled carbohydrate-derived isotopomers by gas chromatography-mass spectrometry (GC-MS). The techniques described may provide an alternative approach for determining the in vitro prebiotic potential of dietary oligosaccharides.
引用
收藏
页码：95 / 98
页数：4
相关论文
共 50 条
[41]   The role of derivatization techniques in the analysis of plant cannabinoids by gas chromatography mass spectrometry [J].
Fodor, B. ;
Molnar-Perl, I. .
TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2017, 95 :149-158
[42]   Recombinant Factor C (rFC) Assay and Gas Chromatography/Mass Spectrometry (GC/MS) Analysis of Endotoxin Variability in Four Agricultural Dusts [J].
Saito, Rena ;
Cranmer, Brian K. ;
Tessari, John D. ;
Larsson, Lennart ;
Mehaffy, John M. ;
Keefe, Thomas J. ;
Reynolds, Stephen J. .
ANNALS OF OCCUPATIONAL HYGIENE, 2009, 53 (07) :713-722
[43]   Separation of Caffeine from Beverages and Analysis Using Thin-Layer Chromatography and Gas Chromatography-Mass Spectrometry [J].
Torres y Torres, Janelle L. ;
Riley, Shauna L. ;
Lorimor, Steven P. ;
Rhoad, Jonathan S. ;
Caldwell, Benjamin D. ;
Zweerink, Gerald L. ;
Ducey, Michael .
JOURNAL OF CHEMICAL EDUCATION, 2015, 92 (05) :900-902
[44]   Determination of Rocket Kerosene in Soil by Static Headspace Analysis Coupled with Gas Chromatography-Mass Spectrometry [J].
Bolotnik, T. A. ;
Smolenkov, A. D. ;
Smirnov, R. S. ;
Shpigun, O. A. .
MOSCOW UNIVERSITY CHEMISTRY BULLETIN, 2015, 70 (04) :168-174
[45]   Comparative qualitative analysis of nonylphenol isomers by gas chromatography-mass spectrometry combined with chemometric resolution [J].
Wu, Ze-ying ;
Zeng, Zhong-da ;
Marriott, Philip J. .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (49) :7759-7766
[46]   Introducing Students to Gas Chromatography-Mass Spectrometry Analysis and Determination of Kerosene Components in a Complex Mixture [J].
Pacot, Giselle Mae M. ;
Lee, Lyn May ;
Chin, Sung-Tong ;
Marriott, Philip J. .
JOURNAL OF CHEMICAL EDUCATION, 2016, 93 (04) :742-746
[47]   Preanalytical variables for liquid chromatography-mass spectrometry (LC-MS) analysis of human blood specimens [J].
Salvagno, Gian Luca ;
Danese, Elisa ;
Lippi, Giuseppe .
CLINICAL BIOCHEMISTRY, 2017, 50 (10-11) :582-586
[48]   Comprehensive Analysis of the Liquid Fraction of Car Tire Pyrolysis Products by Gas Chromatography-Mass Spectrometry [J].
Sholokhova, A. Yu ;
Shashkov, M., V ;
Patrushev, Yu, V ;
Matyushin, D. D. ;
Zhdanov, A. A. ;
Dolgushev, P. A. ;
Buryak, A. K. .
RUSSIAN JOURNAL OF APPLIED CHEMISTRY, 2021, 94 (01) :122-128
[49]   Micro-bore column fast gas chromatography-mass spectrometry in essential oil analysis [J].
Tranchida, Peter Quinto ;
Costa, Rosaria ;
Dugo, Paola ;
Dugo, Giovanni ;
Mondello, Luigi .
NATURAL PRODUCT COMMUNICATIONS, 2008, 3 (07) :1161-1164
[50]   Analysis of Pesticides in Plant Foods by QuEChERS and Gas Chromatography-Mass Spectrometry: An Undergraduate Laboratory Experiment [J].
Hengel, Matt J. ;
Wong, Jon W. ;
Redman, Zachary C. ;
Rering, Caitlin ;
Williams, Katryn L. .
JOURNAL OF CHEMICAL EDUCATION, 2020, 97 (01) :226-233
12345