Gas chromatography-mass spectrometry (GC-MS) techniques for metabolic flux analysis of the Bifido shunt pathway

被引：3
作者：
Price, Neil P.
Whitehead, Terence R.
Cote, Gregory L.
机构：
[1] USDA ARS, Natl Ctr Agr Utilizat Res, Bioprod & Biocatalysis Res Unit, Peoria, IL 61604 USA
[2] USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biotechnol Res Unit, Peoria, IL 61604 USA
来源：
BIOCATALYSIS AND BIOTRANSFORMATION | 2006年 / 24卷 / 1-2期
关键词：
bifidobacterium; bifido shunt; flux; mass spectrometry; metabolism;
D O I：
10.1080/10242420500533325
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The presence of the Bifido shunt in Bifidobacterium is predicted to lead to the uptake and metabolic conversion of fructose to acetate and lactate. We propose an approach to quantifying the carbon flux through the Bifido shunt by measuring specific C-13-labeled carbohydrate-derived isotopomers by gas chromatography-mass spectrometry (GC-MS). The techniques described may provide an alternative approach for determining the in vitro prebiotic potential of dietary oligosaccharides.
引用
收藏
页码：95 / 98
页数：4
相关论文
共 50 条
[31]   Modem developments in gas chromatography-mass spectrometry-based environmental analysis [J].
Santos, FJ ;
Galceran, MT .
JOURNAL OF CHROMATOGRAPHY A, 2003, 1000 (1-2) :125-151
[32]   Global analysis of multiple gas chromatography-mass spectrometry (GC/MS) data sets: A method for resolution of co-eluting components with comparison to MCR-ALS [J].
van Stokkum, Ivo H. M. ;
Mullen, Katharine M. ;
Mihaleva, Velitchka V. .
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS, 2009, 95 (02) :150-163
[33]   Use of hydrogen as a carrier gas for the analysis of steroids with anabolic activity by gas chromatography-mass spectrometry [J].
Munoz-Guerra, J. A. ;
Prado, P. ;
Garcia-Tenorio, S. Vargas .
JOURNAL OF CHROMATOGRAPHY A, 2011, 1218 (41) :7365-7370
[34]   Quantitative Analysis of Tetramethylenedisulfotetramine (Tetramine) Spiked into Beverages by Liquid Chromatography-Tandem Mass Spectrometry with Validation by Gas Chromatography-Mass Spectrometry [J].
Owens, Janel ;
Hok, Saphon ;
Alcaraz, Armando ;
Koester, Carolyn .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (10) :4058-4067
[35]   Exact mass GC-MS analysis: Protocol, database, advantages and application to plant metabolic profiling [J].
Abadie, Cyril ;
Lalande, Julie ;
Tcherkez, Guillaume .
PLANT CELL AND ENVIRONMENT, 2022, 45 (10) :3171-3183
[36]   Comparative Analysis of Amino Acids from Arabidopsis Wild-Type and Mutant sir1-1 Leaves by Reverse-Phase High-Pressure Liquid Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS) [J].
Samami, Arman Allboje ;
Nunes-Nesi, Adriano ;
Wirtz, Markus ;
Hell, Ruediger .
SULFUR METABOLISM IN PLANTS: MECHANISMS AND APPLICATIONS TO FOOD SECURITY AND RESPONSES TO CLIMATE CHANGE, 2012, :103-107
[37]   Gas Chromatography-Mass Spectrometry Analysis of High-Purity Volatile Inorganic Hydrides [J].
Sozin, A. Yu ;
Krylov, V. A. ;
Chernova, O. Yu ;
Sorochkina, T. G. ;
Kotkov, A. P. ;
Grishnova, N. D. ;
Skosyrev, A., I ;
Pushkarev, G., V .
JOURNAL OF ANALYTICAL CHEMISTRY, 2021, 76 (05) :535-545
[38]   Analysis fatty acids profile in Tabanus bivittatus mats with gas chromatography-mass spectrometry [J].
Yanhua, Wang ;
Fuhua, Wu ;
Zhaohan, Guo ;
Mingxing, Peng ;
Min, Xia ;
Zhenling, Pang ;
Xiaoli, Wang ;
Zian, Liang ;
Naiqun, Zhang .
Open Biotechnology Journal, 2015, 9 (01) :113-118
[39]   A review of strategies for untargeted urinary metabolomic analysis using gas chromatography-mass spectrometry [J].
Khodadadi, Mohammad ;
Pourfarzam, Morteza .
METABOLOMICS, 2020, 16 (06)
[40]   Gas Chromatography-Mass Spectrometry Analysis of Cannabis: Undergraduate Organic Chemistry Laboratory Exercise [J].
Lynch, Hailey N. ;
Authement, Claire Chaz ;
Maczko, Autumn ;
Parker, Miranda ;
Beaty, Kaylan ;
Pathiranage, Anuradha Liyana .
JOURNAL OF CHEMICAL EDUCATION, 2023, 100 (03) :1303-1312
12345