Gas chromatography-mass spectrometry (GC-MS) techniques for metabolic flux analysis of the Bifido shunt pathway

被引：3
作者：
Price, Neil P.
Whitehead, Terence R.
Cote, Gregory L.
机构：
[1] USDA ARS, Natl Ctr Agr Utilizat Res, Bioprod & Biocatalysis Res Unit, Peoria, IL 61604 USA
[2] USDA ARS, Natl Ctr Agr Utilizat Res, Fermentat Biotechnol Res Unit, Peoria, IL 61604 USA
来源：
BIOCATALYSIS AND BIOTRANSFORMATION | 2006年 / 24卷 / 1-2期
关键词：
bifidobacterium; bifido shunt; flux; mass spectrometry; metabolism;
D O I：
10.1080/10242420500533325
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The presence of the Bifido shunt in Bifidobacterium is predicted to lead to the uptake and metabolic conversion of fructose to acetate and lactate. We propose an approach to quantifying the carbon flux through the Bifido shunt by measuring specific C-13-labeled carbohydrate-derived isotopomers by gas chromatography-mass spectrometry (GC-MS). The techniques described may provide an alternative approach for determining the in vitro prebiotic potential of dietary oligosaccharides.
引用
收藏
页码：95 / 98
页数：4
相关论文
共 50 条
[21]   Quantitative Analysis of Bisphenol A Leached from Household Plastics by Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry (SPME-GC-MS) [J].
Johnson, Bettie Obi ;
Burke, Femanda M. ;
Harrison, Rebecca ;
Burdette, Samantha .
JOURNAL OF CHEMICAL EDUCATION, 2012, 89 (12) :1555-1560
[22]   Enhanced gas chromatography-mass spectrometry method for bacterial polyhydroxyalkanoates analysis [J].
Tan, Giin-Yu Amy ;
Chen, Chia-Lung ;
Ge, Liya ;
Li, Ling ;
Wang, Lin ;
Zhao, Lei ;
Mo, Yu ;
Tan, Swee Ngin ;
Wang, Jing-Yuan .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2014, 117 (03) :379-382
[23]   Gas Chromatography Tandem Mass Spectrometry (GC-MS/MS) for High-Throughput Screening of Pesticides in Rice Samples Collected in Bangladesh and Saudi Arabia [J].
Strashnov, Ilya ;
Ahmed, Farah T. ;
Alrashdi, May M. ;
Nesmiyan, Inna ;
Polya, David A. .
PROCESSES, 2024, 12 (10)
[24]   Targeted mass spectral ratio analysis: A new tool for gas chromatography-mass spectrometry [J].
Kehimkar, Benjamin ;
Hoggard, Jamin C. ;
Nadeau, Jeremy S. ;
Synovec, Robert E. .
TALANTA, 2013, 103 :267-275
[25]   Determination of procymidone in sicilian olive oils by gas chromatography-mass spectrometry (HRGC-MS) [J].
Dugo, G. ;
Potorti, A. G. ;
Fotia, V. ;
Saitta, M. ;
Lo Turco, V. .
RIVISTA ITALIANA DELLE SOSTANZE GRASSE, 2010, 87 (04) :219-225
[26]   An alternative derivatization method for the analysis of amino acids in cerebrospinal fluid by gas chromatography-mass spectrometry [J].
Nunes de Paiva, Maria Jose ;
Menezes, Helvecio Costa ;
Christo, Paulo Pereira ;
Resende, Rodrigo Ribeiro ;
Cardeal, Zenilda de Lourdes .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2013, 931 :97-102
[27]   Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) for Rapid Determination of Microbial Cell Lipid Content: Correlation with Gas Chromatography-Mass Spectrometry (GC-MS) [J].
Millan-Oropeza, Aaron ;
Rebois, Rolando ;
David, Michelle ;
Moussa, Fathi ;
Dazzi, Alexandre ;
Bleton, Jean ;
Virolle, Marie-Joelle ;
Deniset-Besseau, Ariane .
APPLIED SPECTROSCOPY, 2017, 71 (10) :2344-2352
[28]   Gas chromatography-mass spectrometry analysis of β2-agonists in bovine retina [J].
Hernández-Carrasquilla, M .
ANALYTICA CHIMICA ACTA, 2000, 408 (1-2) :285-290
[29]   Quantitative analysis of tropane alkaloids in biological materials by gas chromatography-mass spectrometry [J].
Namera, A ;
Yashiki, M ;
Hirose, Y ;
Yamaji, S ;
Tani, T ;
Kojima, T .
FORENSIC SCIENCE INTERNATIONAL, 2002, 130 (01) :34-43
[30]   Isotope-Dilution Gas Chromatography-Mass Spectrometry Method for the Analysis of Hydroxyurea [J].
Garg, Uttam ;
Scott, David ;
Frazee, Clint ;
Kearns, Gregory ;
Neville, Kathleen .
THERAPEUTIC DRUG MONITORING, 2015, 37 (03) :325-330
12345