Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways

被引：30

作者：

Duan, Hu ^{[1
,2
]}

Jin, Songjun ^{[1
]}

Zhang, Yan ^{[1
]}

Li, Fuhua ^{[1
]}

Xiang, Jianhai ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2014年 / 46卷 / 02期

关键词：

Granulocyte; Endocytosis; Beads; E; coli; WSSV; Cherax quadricarinatus; CARIBBEAN SPINY LOBSTER; CELL-MEDIATED-IMMUNITY; CIRCULATING HEMOCYTES; VIRUS ENTRY; FUSION; PATHWAY; NUCLEOPOLYHEDROVIRUS; HEMATOPOIESIS; INFECTION; SHRIMP;

D O I：

10.1016/j.dci.2014.04.006

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The hemocytes of the red claw crayfish Cherax quadricarinatus are classified by morphologic observation into the following types: hyalinocytes (H), semi-granulocytes (SG) and granulocytes (G). Density gradient centrifugation with Percoll was developed to separate these three subpopulations of hemocytes. Beads, Escherichia coli, and FITC labeling WSSV were used to investigate the characteristics of granulocytes by using scanning electron microscope, transmission electron microscope, and laser scan confocal microscope. Results showed that granulocytes could phagocytose beads and E. coli by endocytic pathways. WSSV could rely on caveolae-mediated endocytosis to mainly enter into granulocytes. These results could elucidate the mechanism of the innate immunity function of granulocytes, and it also showed the mechanism by which WSSV invaded granulocytes in the red claw crayfish. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：186 / 193

页数：8

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