Highly sensitive SERS detection and quantification of sialic acid on single cell using photonic-crystal fiber with gold nanoparticles

被引：80

作者：

Gong, Tianxun ^{[1
,2
]}

Cui, Ying ^{[1
,3
]}

Goh, Douglas ^{[2
]}

Voon, Kong Kien ^{[2
]}

Shum, Perry Ping ^{[1
,3
]}

Humbert, Georges ^{[4
]}

Auguste, Jean-Louis ^{[4
]}

Dinh, Xuan-Quyen ^{[3
]}

Yong, Ken-Tye ^{[1
]}

Olivo, Malini ^{[2
,5
]}

机构：

[1] Nanyang Technol Univ, Sch Elect & Elect Engn, Singapore 639798, Singapore

[2] ASTAR, SBIC, Bioopt Imaging Grp, Singapore 138667, Singapore

[3] CINTRA CNRS NTU THALES, Singapore 637553, Singapore

[4] Univ Limoges, CNRS, Xlim UMR 6172, F-87060 Limoges, France

[5] Natl Univ Ireland Univ Coll Galway, Sch Phys, Galway, Ireland

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 64卷

关键词：

Side channel PCF; Sialic acid; 4-(dihydroxyborophenyl)acetylene; Single cell; SERS; Gold nanoparticles; ENHANCED RAMAN-SCATTERING; QUANTITATIVE-DETERMINATION; LIQUID-CHROMATOGRAPHY; PHENYLBORONIC ACID; HELA-CELLS; SURFACE; FLUORESCENCE; EXPRESSION; MICROSCOPY; MOLECULE;

D O I：

10.1016/j.bios.2014.08.077

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An ultrasensitive surface enhanced Raman spectroscopy (SERS) based sensing platform was developed to detect the mean sialic acid level on the surface of single cell with sensitivity as low as 2 fmol. This platform adopted the use of an interference-free Raman tag, 4-(dihydroxyborophenyl) acetylene (DBA), which selectively binds to sialic acid on the cell membrane. By loading the side channel of a photonic crystal fiber with a mixture of gold nanoparticles and DBA-tagged HeLa cell, and subsequently propagating laser light through the central solid core, strong SERS signal was obtained. This SERS technique achieved accurate detection and quantification of concentration of sialic acid on a single cell, surpassing previously reported methods that required more than 10(5) cells. Moreover, this platform can be developed into a clinical diagnostic tool to potentially analyze sialic acid-related diseases such as tumor malignancy and metastasis in real-time. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：227 / 233

页数：7

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