Phytogenic synthesis of silver nanobactericides for anti-biofilm activity against human pathogen H. pylori

The present study reports the phytogenic synthesis of silver nanobactericides using Acorus calamus L. and their anti-biofilm activity against clinically isolated H. pylori. The synthesis was confirmed with change in the color of the reaction mixture to brown. The increased in the color intensity was periodically monitored with UV-visible spectroscopy which displayed maximum absorption at 410 nm. The biomolecular interaction was studied with FTIR spectral measurements of silver nanobactericides which revealed the presence of broad absorbance band appearing at 3361 is due to OH group and the prominent peak at 1634 correspond to an amide group. X-ray diffraction (XRD) displayed Bragg's intensities at 2 theta angle reflecting (111), (200), (220) and (311) of the face centered cubic (fcc) structure of silver which was compared with standard XRD pattern. The morphological characteristics of nanobactericides were studied using Transmission electron microscopy (TEM) analysis which revealed the polydispersity of nanoparticles with size ranging from 5 to 60 nm. The anti-biofilm activity of silver nanobactericides against H. pylori was measured using crystal violet and ruthenium red assays which revealed 350 mu g/mL to be more effective. The obtained activity was validated with standard antibiotics amoxicillin. Overall, the results obtained in the present investigation are promising enough to reveal the efficacy of silver nanoparticles to inhibit the biofilm production.