Effect of allopurinol on the kidney function, histology and injury biomarker (NGAL, IL-18) levels in uninephrectomised rats subjected to ischaemia-reperfusion injury

被引：11

作者：

Bussmann, Andre Roberto ^{[1
]}

Marton Filho, Marcos Antonio ^{[2
]}

Modolo, Marilia Pinheiro ^{[2
]}

Modolo, Renata Pinheiro ^{[3
]}

Amado, Patricia ^{[2
]}

Custodio Domingues, Maria Aparecida ^{[4
]}

Machado Castiglia, Yara Marcondes ^{[5
]}

Pinheiro Modolo, Norma Sueli ^{[5
]}

机构：

[1] UNESP, Botucatu Med Sch, Postgrad Program Anaesthesiol, BR-18618970 Botucatu, SP, Brazil

[2] UNESP, Botucatu Med Sch, BR-18618970 Botucatu, SP, Brazil

[3] ABC, Sch Med, Santo Andre, SP, Brazil

[4] UNESP, Botucatu Med Sch, Dept Pathol, BR-18618970 Botucatu, SP, Brazil

[5] UNESP, Botucatu Med Sch, Dept Anaesthesiol, BR-18618970 Botucatu, SP, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2014年 / 29卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

Ischemia; Acute Kidney Injury; Allopurinol; Interleukin-18; Biomarkers; Pharmacological; Rats; CRITICALLY-ILL CHILDREN; ACUTE-RENAL-FAILURE; CONSENSUS CONFERENCE; LIPOCALIN NGAL; DIAGNOSIS; URINARY; MODEL; OUTCOMES;

D O I：

10.1590/S0102-86502014000800006

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

PURPOSE: To investigate whether allopurinol exerts a protective effect on kidneys by measuring new kidney injury biomarkers (NGALp, NGALu, KIM-1 and IL-18) and analysing the renal function and histology in uninephrectomised rats subjected to ischaemia-reperfusion injury. METHODS: Thirty two Wistar rats were randomly allocated to four groups: Sham (S): laparotomy; Control (C): laparotomy and ischaemia-reperfusion in the left kidney; Control Allopurinol (CA): laparotomy and allopurinol at a dose of 100mg.kg(-1).d(-1); and Allopurinol (A): laparotomy ischaemia-reperfusion in the left kidney and allopurinol at a dose of 100mg.kg(-1).d(-1). The NGALp, NGALu, KIM-1, IL-18 and creatinine levels and the kidney histology were analysed. The significance level was established as p<0.05. RESULTS: Creatinine level increased in all the groups, with A approximate to C > S approximate to CA. The NGALp, NGALu and IL-18 levels exhibited similar behaviour in all the groups. KIM-1 was higher in group A than C and showed intermediate values in groups S and CA. Severity of injury in the left kidney was greater in groups C and A compared to S and CA. CONCLUSION: Allopurinol did not exert protective or damaging effects on the kidneys of rats subjected to ischaemia-reperfusion injury.

引用

页码：515 / 521

页数：7

共 20 条

[1] Acute renal failure - definition, outcome measures, animal models, fluid therapy and information technology needs: the Second International Consensus Conference of the Acute Dialysis Quality Initiative (ADQI) Group [J].

Bellomo, R ;

Ronco, C ;

Kellum, JA ;

Mehta, RL ;

Palevsky, P .

CRITICAL CARE, 2004, 8 (04) :R204-R212

[2] Update on mechanisms of ischemic acute kidney injury [J].

Devarajan, Prasad .

JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2006, 17 (06) :1503-1520

[3] The predictive performance of plasma neutrophil gelatinase-associated lipocalin (NGAL) increases with grade of acute kidney injury [J].

Haase-Fielitz, Anja ;

Bellomo, Rinaldo ;

Devarajan, Prasad ;

Bennett, Michael ;

Story, David ;

Matalanis, George ;

Frei, Ulrich ;

Dragun, Duska ;

Haase, Michael .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2009, 24 (11) :3349-3354

[4] Protective Effects of Reducing Renal Ischemia-reperfusion Injury During Renal Hilar Clamping: Use of Allopurinol as a Nephroprotective Agent [J].

Keel, Christopher E. ;

Wang, Zijun ;

Colli, Janet ;

Grossman, Leah ;

Majid, Dewan ;

Lee, Benjamin R. .

UROLOGY, 2013, 81 (01) :210.e5-210.e10

[5]

Lee BE, 2009, J INVEST MED, V57, P902, DOI 10.2310/JIM.0b013e3181bca50c

[6] Isoflurane protects against renal ischemia and reperfusion injury and modulates leukocyte infiltration in mice [J].

Lee, H. Thomas ;

Kim, Mihwa ;

Kim, Minjae ;

Kim, Nala ;

Billings, Frederic T. ;

D'Agati, Vivette D. ;

Emala, Charles W., Sr. .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2007, 293 (03) :F713-F722

[7] Diagnosis of Acute Kidney Injury Using Functional and Injury Biomarkers: Workgroup Statements from the Tenth Acute Dialysis Quality Initiative Consensus Conference [J].

McCullough, Peter A. ;

Shaw, Andrew D. ;

Haase, Michael ;

Bouchard, Josee ;

Waikar, Sushrut S. ;

Siew, Edward D. ;

Murray, Patrick T. ;

Mehta, Ravindra L. ;

Ronco, Claudio .

ADQI CONSENSUS ON AKI BIOMARKERS AND CARDIORENAL SYNDROMES, 2013, 182 :13-+

[8] Acute Kidney Injury Network: report of an initiative to improve outcomes in acute kidney injury [J].

Mehta, Ravindra L. ;

Kellum, John A. ;

Shah, Sudhir V. ;

Molitoris, Bruce A. ;

Ronco, Claudio ;

Warnock, David G. ;

Levin, Adeera .

CRITICAL CARE, 2007, 11 (02)

[9] Acute renal ischemia model in dogs:: Effects of metoprolol [J].

Módolo, NSP ;

Castiglia, YMM ;

Ganem, EM ;

Braz, JRC ;

Vianna, PTG ;

Vane, LA .

RENAL FAILURE, 2001, 23 (01) :1-10

[10] Some biomarkers of acute kidney injury are increased in pre-renal acute injury [J].

Nejat, Maryam ;

Pickering, John W. ;

Devarajan, Prasad ;

Bonventre, Joseph V. ;

Edelstein, Charles L. ;

Walker, Robert J. ;

Endre, Zoltan H. .

KIDNEY INTERNATIONAL, 2012, 81 (12) :1254-1262

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