Expression of hypoxia-inducible factor-1α, endothelin-1 and adrenomedullin in newborn rats with hypoxia-induced pulmonary hypertension

被引:20
作者
Wang, Le [1 ]
Zhou, Ying [1 ]
Li, Mingxia [1 ]
Zhu, Yanping [1 ]
机构
[1] Xinjiang Med Univ, Dept Neonatol, Affiliated Hosp 1, Urumqi 830011, Xinjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
hypoxia-inducible factor-1; endothelin-1; adrenomedullin; pulmonary hypertension; rat; HIF-1-ALPHA; PATHWAY; ARTERY; GENE;
D O I
10.3892/etm.2014.1728
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Hypoxia-inducible factor (HIF)-1 alpha is associated with hypoxia-induced pulmonary hypertension (HPH) in adults. In the present study, the expression levels of HIF-1 alpha, endothelin (ET)-1 and adrenomedullin (ADM) were analyzed during HPH in neonates. In total, 96 newborn rats were subjected to hypoxia or normoxia for 3, 5, 7, 10, 14 or 21 days (n=8 per subgroup). HIP-1 alpha, ET-1 and ADM expression levels were measured by quantitative polymerase chain reaction. In addition, the intima-media thickness/external diameter ratio (MT%) and medial wall cross-sectional area/vessel total cross-sectional area ratio (MA%) were calculated to evaluate pulmonary vascular remodeling. The mean pulmonary arterial pressure (mPAP) increased with exposure to hypoxia. Furthermore, the expression levels of HIF-1 alpha, ET-1 and ADM in the lungs were shown to increase after three and five days of hypoxia, while the MT% and MA% increased after seven days of hypoxia, as compared with the controls (P<0.05). Therefore, the expression of HIF-1 alpha, ET-1 and ADM is upregulated in the lungs of newborn rats during early HPH. At later stages, the mPAP increases, vascular remodeling occurs and HIP-1 alpha, ET-1 and ADM expression levels restore to normal levels.
引用
收藏
页码:335 / 339
页数:5
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