Interaction studies of sodium cyclamate with DNA revealed by spectroscopy methods

The interaction between sodium cyclamate (SC) and calf thymus DNA in simulated physiological buffer (pH 7.4) using ethidium bromide (EB) as fluorescence probe was investigated by UV-vis spectrometry (UV), fluorescence, resonance light scattering (RLS) and Fourier transform infrared (FT-IR) spectroscopy, along with DNA melting studies and cyclic voltammetric (CV) measurements. The results indicate that SC can not only bind into the minor groove of DNA, but also intercalate into the DNA Base pairs. Based on UV data, the binding constant K and binding sites n of the formed DNA/SC complex were estimated to be 2.83 x 10(3) mol/L and 2.0, respectively. Fluorescence results demonstrate that the quenching of DNA/EB induced by SC can mainly be attributed to static procedure. The melting studies and CV analysis further confirm that the interaction mechanism between the SC and DNA is similar to that of DNA intercalator. The results of FT-IR spectra show that a specific interaction mainly exist between SC and adenine and guanine bases of DNA, which resulting in potential damage due to some change in the information structure. The DNA saturation binding value estimated to be 1.67 based on the RLS data also indicated that SC may cause damage of DNA. (C) 2019 Elsevier B.V. All rights reserved.