Human anti-rhesus D IgG1 antibody produced in transgenic plants

被引：28
作者：
Bouquin, T
Thomsen, M
Nielsen, LK
Green, TH
Mundy, J
Dziegiel, MH
机构：
[1] Univ Copenhagen Hosp, HS Blodbanken, DK-2100 Copenhagen O, Denmark
[2] Univ Copenhagen, Inst Mol Biol, Dept Plant Physiol, DK-1353 Copenhagen, Denmark
来源：
TRANSGENIC RESEARCH | 2002年 / 11卷 / 02期
关键词：
alloimmunization; immunoglobulin; Rhesus; transgenic plants;
D O I：
10.1023/A:1015226418688
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Transgenic plants represent an alternative to cell culture systems for producing cheap and safe antibodies for diagnostic and therapeutic use. To evaluate the functional properties of a 'plantibody', we generated transgenic Arabidopsis plants expressing full-length human IgG1 against the Rhesus D antigen, which is responsible for alloimmunization of RhD- mothers carrying an RhD+ fetus. Anti-RhD extracted from plants specifically reacted with RhD+ cells in antiglobulin technique, and elicited a respiratory burst in human peripheral blood mononuclear cells. Plant-derived antibody had equivalent properties to CHO cell-produced anti-RhD antibody, indicating its potential usefulness in diagnostic and therapeutic programs.
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收藏
页码：115 / 122
页数：8
相关论文
共 34 条
[1]   Galactose-extended glycans of antibodies produced by transgenic plants [J].
Bakker, H ;
Bardor, M ;
Molthoff, JW ;
Gomord, V ;
Elbers, I ;
Stevens, LH ;
Jordi, W ;
Lommen, A ;
Faye, L ;
Lerouge, P ;
Bosch, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (05) :2899-2904
[2]  
BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194
[3]   COMBINATORIAL AND SYNERGISTIC PROPERTIES OF CAMV 35S-ENHANCER SUBDOMAINS [J].
BENFEY, PN ;
REN, L ;
CHUA, NH .
EMBO JOURNAL, 1990, 9 (06) :1685-1696
[4]   N-glycosylation of a mouse IgG expressed in transgenic tobacco plants [J].
Cabanes-Macheteau, M ;
Fitchette-Lainé, AC ;
Loutelier-Bourhis, C ;
Lange, C ;
Vine, ND ;
Ma, JKC ;
Lerouge, P ;
Faye, L .
GLYCOBIOLOGY, 1999, 9 (04) :365-372
[5]   A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice [J].
Chargelegue, D ;
Vine, ND ;
van Dolleweerd, CJ ;
Drake, PMW ;
Ma, JKC .
TRANSGENIC RESEARCH, 2000, 9 (03) :187-194
[6]   Gene silencing results in instability of antibody production in transgenic plants [J].
De Neve M. ;
De Buck S. ;
De Wilde C. ;
Van Houdt H. ;
Strobbe I. ;
Jacobs A. ;
Van Montagu M. ;
Depicker A. .
Molecular and General Genetics MGG, 1999, 260 (6) :582-592
[7]   PHAGE DISPLAY USED FOR GENE CLONING OF HUMAN RECOMBINANT ANTIBODY AGAINST THE ERYTHROCYTE SURFACE-ANTIGEN, RHESUS-D [J].
DZIEGIEL, M ;
NIELSEN, LK ;
ANDERSEN, PS ;
BLANCHER, A ;
DICKMEISS, E ;
ENGBERG, J .
JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 182 (01) :7-19
[8]   Molecular farming of pharmaceutical proteins [J].
Fischer, R ;
Emans, N .
TRANSGENIC RESEARCH, 2000, 9 (4-5) :279-299
[9]   N-glycans harboring the Lewis a epitope are expressed at the surface of plant cells [J].
Fitchette-Lainé, AC ;
Gomord, V ;
Cabanes, M ;
Michalski, JC ;
Saint Macary, M ;
Foucher, B ;
Cavelier, B ;
Hawes, C ;
Lerouge, P ;
Faye, L .
PLANT JOURNAL, 1997, 12 (06) :1411-1417
[10]   Transgenic plants as factories for biopharmaceuticals [J].
Giddings, G ;
Allison, G ;
Brooks, D ;
Carter, A .
NATURE BIOTECHNOLOGY, 2000, 18 (11) :1151-1155
1234