An integrated in vitro model of perfused tumor and cardiac tissue

被引:52
作者
Moya, Monica [1 ,2 ]
Tran, David [2 ,3 ]
George, Steven C. [1 ,2 ,3 ,4 ]
机构
[1] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA 92697 USA
[2] Univ Calif Irvine, Edwards Lifesci Ctr Adv Cardiovasc Technol, Irvine, CA 92697 USA
[3] Univ Calif Irvine, Dept Chem Engn & Mat Sci, Irvine, CA 92697 USA
[4] Univ Calif Irvine, Dept Med, Irvine, CA 92697 USA
来源
STEM CELL RESEARCH & THERAPY | 2013年 / 4卷
基金
美国国家卫生研究院;
关键词
cardiomyocyte; microcirculation; induced pluripotent stem cell; microphysiological system; cancer; HOST VASCULATURE; STEM-CELLS; MICROENVIRONMENT; ANGIOGENESIS; MICROSCOPY; DENSITY; GROWTH; CARDIOMYOCYTES; PROGRESSION; FIBROBLASTS;
D O I
10.1186/scrt376
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Cancer and cardiovascular disease remain the two leading causes of death in the United States. Progress in treatment to reduce morbidity and mortality will include the development of new drugs. Recent advances in induced pluripotent stem cell technology, tissue engineering, and microfabrication techniques have created a unique opportunity to develop three-dimensional (3D) microphysiological systems that more accurately reflect in vivo human biology when compared with two-dimensional flat systems or animal models. Our group is working to develop 3D microphysiological systems using induced pluripotent stem cell technology that simulates the microcirculation, the cardiac muscle, and the solid tumor, and then to combine these systems into an integrated microphysiological system that simulates perfused cardiac muscle and solid tumor on a single platform. The platform will be initially validated to predict anti-cancer efficacy while minimizing cardiac muscle toxicity. A critical feature will be blood flow through a human microcirculation (capillaries and larger microvessels), which is necessary to overcome diffusion limitations of nutrients and waste products in realistic 3D cultures, and serves to integrate multiple organ systems. This is a necessary and critical feature of any platform that seeks to simulate integrated human organ systems. The results of our project should produce a new paradigm for efficient and accurate drug and toxicity screening, initially for anti-cancer drugs with minimal cardiac side effects, and a platform technology that can be eventually used to integrate multiple major organ systems of the human body.
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页数:6
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