Characterization and cDNA cloning of androgenic gland hormone of the terrestrial isopod Armadillidium vulgare

被引：97

作者：

Okuno, A

Hasegawa, Y

Ohira, T

Katakura, Y

Nagasawa, H ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Aquat Biosci, Bunkyo Ku, Tokyo 1138657, Japan

[3] Keio Univ, Dept Biol, Setagaya Ku, Yokohama, Kanagawa 2238521, Japan

[4] Todoroki, Setagaya Ku, Tokyo 1580082, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 264卷 / 02期

关键词：

D O I：

10.1006/bbrc.1999.1522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sex differentiation in crustaceans is known to be controlled by a peptide hormone called androgenic gland hormone (AGH). AGH was extracted and purified from the androgenic glands (AGs) of the male isopod Armadillidium vulgare by high-performance liquid chromatography, AGH consisted of two peptide chains and their N-terminal amino acid sequences were determined. A cDNA encoding AGH was cloned by PCR and sequenced. The cDNA had an open reading frame of 432 bp, which encoded a preproAGH consisting of a signal peptide (21 residues), B chain (44 residues), C peptide (46 residues), and A chain (29 residues). Through processing, the A and B chains might form a heterodimer interlinked by disulfide bonds. The A chain possessed a putative N-linked glycosylation site. A Northern blot analysis using the cDNA as a probe detected a hybridization signal with 0.8 kb in the RNA preparation only from the AGs. (C) 1999 Academic Press.

引用

页码：419 / 423

页数：5

共 19 条

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