Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging

被引：80

作者：

Nakamura, Akinobu ^{[1
,2
]}

Takigawa, Kazumasa ^{[1
]}

Kurishita, Yasutaka ^{[3
]}

Kuwata, Keiko ^{[4
]}

Ishida, Manabu ^{[5
]}

Shimoda, Yasushi ^{[1
]}

Hamachi, Itaru ^{[3
,6
]}

Tsukiji, Shinya ^{[1
,5
]}

机构：

[1] Nagaoka Univ Technol, Dept Bioengn, Nagaoka, Niigata 9402188, Japan

[2] Nagaoka Univ Technol, Dept Elect Engn, Nagaoka, Niigata 9402188, Japan

[3] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Katsura, Kyoto 6158510, Japan

[4] Nagoya Univ, Inst Transformat BioMol ITbM, Chikusa Ku, Nagoya, Aichi 4648602, Japan

[5] Nagaoka Univ Technol, Top Runner Incubat Ctr Acad Ind Fus, Nagaoka, Niigata 9402188, Japan

[6] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Chiyoda Ku, Tokyo 1020075, Japan

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 46期

关键词：

RESONANCE ENERGY-TRANSFER; LIVING MAMMALIAN-CELLS; OXIDATIVE STRESS; DNA-PROBE; IN-VIVO; BINDING; LOCALIZATION; COMPLEX;

D O I：

10.1039/c4cc01753f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.

引用

页码：6149 / 6152

页数：4

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