Global Distribution of Human-Associated Fecal Genetic Markers in Reference Samples from Six Continents

被引：73

作者：

Mayer, Rene E. ^{[1
,2
]}

Reischer, Georg H. ^{[1
,3
]}

Ixenmaier, Simone K. ^{[1
,2
]}

Derx, Julia ^{[2
,4
]}

Blaschke, Alfred Paul ^{[2
,4
]}

Ebdon, James E. ^{[5
]}

Linke, Rita ^{[1
,2
]}

Egle, Lukas ^{[6
]}

Ahmed, Warish ^{[7
]}

Blanch, Anicet R. ^{[8
]}

Byamukama, Denis ^{[9
]}

Savill, Marion ^{[10
]}

Mushi, Douglas ^{[11
]}

Cristobal, Hector A. ^{[12
,13
]}

Edge, Thomas A. ^{[14
]}

Schade, Margit A. ^{[15
]}

Aslan, Asli ^{[16
]}

Brooks, Yolanda M. ^{[17
]}

Sommer, Regina ^{[2
,18
]}

Masago, Yoshifumi ^{[19
]}

Sato, Maria I. ^{[20
]}

Taylor, Huw D. ^{[5
]}

Rose, Joan B. ^{[17
]}

Wuertz, Stefan ^{[21
,22
]}

Shanks, Orin C. ^{[23
]}

Piringer, Harald ^{[24
]}

Mach, Robert L. ^{[25
]}

Savio, Domenico ^{[26
]}

Zessner, Matthias ^{[6
]}

Farnleitner, Andreas H. ^{[1
,2
,26
]}

机构：

[1] TU Wien, Inst Chem Environm & Biosci Engn, Res Grp Environm Microbiol & Mol Diagnost 166 S 3, A-1060 Vienna, Austria

[2] Interuniv Cooperat Ctr Water & Hlth, Vienna, Austria

[3] TU Wien, Inst Chem Environm & Biosci Engn, IFA Tulln, Mol Diagnost Grp, A-3430 Tulln, Austria

[4] TU Wien, Inst Hydraul Engn & Water Resources Management, A-1040 Vienna, Austria

[5] Univ Brighton, Sch Environm & Technol, Environm & Publ Hlth Res & Enterprise Grp, Brighton BN2 4GJ, E Sussex, England

[6] TU Wien, Inst Water Qual & Resource Management, A-1040 Vienna, Austria

[7] CSIRO Land & Water, Ecosci Precinct, 41 Boggo Rd, Brisbane, Qld 4102, Australia

[8] Univ Barcelona, Dept Genet Microbiol & Stat, E-08028 Barcelona, Spain

[9] Makerere Univ, Dept Biochem, POB 27755, Kampala, Uganda

[10] Affordable Water Ltd, Auckland 1011, New Zealand

[11] Sokoine Univ Agr, Dept Biosci, POB 3038, Morogoro, Tanzania

[12] Consejo Nacl Invest Cient & Tecn, Inst Invest Ind Quim INIQUI, Lab Aguas & Suelos, RA-4400 Salta, Argentina

[13] Univ Nacl Salta, RA-4400 Salta, Argentina

[14] Canada Ctr Inland Waters, Environm & Climate Change Canada, Burlington, ON L7S 1A1, Canada

[15] Bavarian Environm Agcy, D-86179 Augsburg, Germany

[16] Georgia Southern Univ, Dept Epidemiol & Environm Hlth Sci, Statesboro, GA 30460 USA

[17] Michigan State Univ, Dept Fisheries & Wildlife, E Lansing, MI 48824 USA

[18] Med Univ Vienna, Inst Hyg & Appl Immunol, Water Hyg, A-1090 Vienna, Austria

[19] Tohoku Univ, New Ind Creat Hatchery Ctr, Sendai, Miyagi 9808579, Japan

[20] CETESB Cia Ambiental Estado Sao Paulo, Dept Anal Ambientais, BR-05459900 Sao Paulo, Brazil

[21] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551, Singapore

[22] Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore 637551, Singapore

[23] US EPA, Off Res & Dev, Cincinnati, OH 45268 USA

[24] VRVis Res Ctr, A-1220 Vienna, Austria

[25] TU Wien, Inst Chem Environm & Biosci Engn, Res Div Biochem Technol, A-1060 Vienna, Austria

[26] Karl Landsteiner Univ Hlth Sci, Dept Pharmacol Physiol & Microbiol, Div Water Qual & Hlth, A-3500 Krems An Der Donau, Austria

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2018年 / 52卷 / 09期

基金：

奥地利科学基金会;

关键词：

MICROBIAL SOURCE TRACKING; 16S RIBOSOMAL-RNA; PCR-BASED ASSAYS; WATER-RESOURCES; WASTE-WATER; QUANTITATIVE DETECTION; RISK-ASSESSMENT; HUMAN SEWAGE; POLLUTION; PERFORMANCE;

D O I：

10.1021/acs.est.7b04438

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Numerous bacterial genetic markers are available for the molecular detection of human sources of fecal pollution in environmental waters. However, widespread application is hindered by a lack of knowledge regarding geographical stability, limiting implementation to a small number of well-characterized regions. This study investigates the geographic distribution of five human-associated genetic markers (HF183/BFDrev, HF183/BacR287, BacHum-UCD, BacH, and Lachno2) in municipal wastewaters (raw and treated) from 29 urban and rural wastewater treatment plants (750-4 400 000 population equivalents) from 13 countries spanning six continents. In addition, genetic markers were tested against 280 human and nonhuman fecal samples from domesticated, agricultural and wild animal sources. Findings revealed that all genetic markers are present in consistently high concentrations in raw (median logio 7.2-8.0 marker equivalents (ME) 100 mL(-1)) and biologically treated wastewater samples (median log(10) 4.6-6.0 ME 100 mL(-1)) regardless of location and population. The false positive rates of the various markers in nonhuman fecal samples ranged from 5% to 47%. Results suggest that several genetic markers have considerable potential for measuring human-associated contamination in polluted environmental waters. This will be helpful in water quality monitoring, pollution modeling and health risk assessment (as demonstrated by QMRAcatch) to guide target-oriented water safety management across the globe.

引用

页码：5076 / 5084

页数：9

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