Vaccine Candidate Against COVID-19 Based on Structurally Modified Plant Virus as an Adjuvant

被引：10
|
作者：
Kovalenko, Angelina O. [1 ]
Ryabchevskaya, Ekaterina M. [1 ]
Evtushenko, Ekaterina A. [1 ]
Manukhova, Tatiana I. [1 ]
Kondakova, Olga A. [1 ]
Ivanov, Peter A. [1 ]
Arkhipenko, Marina V. [1 ]
Gushchin, Vladimir A. [1 ,2 ]
Nikitin, Nikolai A. [1 ]
Karpova, Olga V. [1 ]
机构：
[1] Lomonosov Moscow State Univ, Fac Biol, Dept Virol, Moscow, Russia
[2] Minist Hlth Russian Federat, NF Gamaleya Natl Res Ctr Epidemiol & Microbiol, Moscow, Russia
来源：
FRONTIERS IN MICROBIOLOGY | 2022年 / 13卷
基金：
俄罗斯基础研究基金会;
关键词：
coronaviruses; vaccine candidate; plant virus; adjuvant; tobacco mosaic virus; structurally modified plant virus; RECEPTOR-BINDING DOMAIN; TOBACCO-MOSAIC-VIRUS; SARS-COV-2; SPIKE; SPHERICAL-PARTICLES; SARS-COV; PROTEIN; MUTATIONS; INFECTION; EPITOPES; VIRIONS;
D O I：
10.3389/fmicb.2022.845316
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A recombinant vaccine candidate has been developed based on the major coronaviruses' antigen (S protein) fragments and a novel adjuvant-spherical particles (SPs) formed during tobacco mosaic virus thermal remodeling. The receptor-binding domain and the highly conserved antigenic fragments of the S2 protein subunit were chosen for the design of recombinant coronavirus antigens. The set of three antigens (Co1, CoF, and PE) was developed and used to create a vaccine candidate composed of antigens and SPs (SPs + 3AG). Recognition of SPs + 3AG compositions by commercially available antibodies against spike proteins of SARS-CoV and SARS-CoV-2 was confirmed. The immunogenicity testing of these compositions in a mouse model showed that SPs improved immune response to the CoF and PE antigens. Total IgG titers against both proteins were 9-16 times higher than those to SPs. Neutralizing activity against SARS-CoV-2 in serum samples collected from hamsters immunized with the SPs + 3AG was demonstrated.
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页数：15
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