Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives

被引:84
作者
Cao, Xu [1 ]
Yakala, Gopala K. [1 ]
van den Hil, Francijna E. [1 ]
Cochrane, Amy [1 ]
Mummery, Christine L. [1 ]
Orlova, Valeria V. [1 ]
机构
[1] Leiden Univ, Med Ctr, Dept Anat & Embryol, Einthovenweg 20, NL-2333 ZC Leiden, Netherlands
基金
欧盟地平线“2020”;
关键词
PLURIPOTENT STEM-CELLS; ENDOTHELIAL-CELLS; PHAGOCYTOSIS; ACTIVATION; MECHANISMS; GENERATION; EXPANSION; PERICYTES;
D O I
10.1016/j.stemcr.2019.05.003
中图分类号
Q813 [细胞工程];
学科分类号
摘要
A renewable source of human monocytes and macrophages would be a valuable alternative to primary cells from peripheral blood (PB) in biomedical research. We developed an efficient protocol to derive monocytes and macrophages from human induced pluripotent stem cells (hiPSCs) and performed a functional comparison with PB-derived cells. hiPSC-derived monocytes were functional after cryopreservation and exhibited gene expression profiles comparable with PB-derived monocytes. Notably, hiPSC-derived monocytes were more activated with greater adhesion to endothelial cells under physiological flow. hiPSC-derived monocytes were successfully polarized to M1 and M2 macrophage subtypes, which showed similar pan- and subtype-specific gene and surface protein expression and cytokine secretion to PB-derived macrophages. hiPSC-derived macrophages exhibited higher endocytosis and efferocytosis and similar bacterial and tumor cell phagocytosis to PB-derived macrophages. In summary, we developed a robust protocol to generate hiPSC monocytes and macrophages from independent hiPSC lines that showed aspects of functional maturity comparable with those from PB.
引用
收藏
页码:1282 / 1297
页数:16
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