Polymerase chain reaction assay to identify all immature stages of two species of the Anopheles quadrimaculatus sibling species complex (Diptera: Culicidae)

被引：0
|
作者：
Rutledge, CR [1 ]
Wesson, DM
Meek, CL
机构：
[1] Louisiana State Univ, Dept Entomol, Baton Rouge, LA 70893 USA
[2] Tulane Univ, Sch Publ Hlth & Trop Med, Dept Trop Med, New Orleans, LA 70126 USA
来源：
JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION | 1999年 / 15卷 / 04期
关键词：
Anopheles quadrimaculatus; An; inundatus; immature stages; polymerase chain reaction;
D O I：
暂无
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
A polymerase chain reaction assay that detects differences in the 2nd internal transcribed spacer of ribosomal DNA was tested as a means to identify all immature stages of 2 species in the Anopheles quadrimaculatus sibling species complex. The technique was successful in amplifying DNA from eggs, 1st-4th instars, and pupae of An. quadrimaculatus Say and An. inundatus Reinert.
引用
收藏
页码：573 / 575
页数：3
相关论文
共 40 条
  • [31] A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species
    Rivas, R
    Velázquez, E
    Valverde, A
    Mateos, PF
    Martínez-Molina, E
    ELECTROPHORESIS, 2001, 22 (06) : 1086 - 1089
  • [32] A population genetics study of Anopheles darlingi (Diptera: Culicidae) from Colombia based on random amplified polymorphic DNA-polymerase chain reaction and amplified fragment lenght polymorphism markers
    Gonzalez, Ranulfo
    Wilkerson, Richard
    Suarez, Marco Fidel
    Garcia, Felipe
    Gallego, Gerardo
    Cardenas, Heiber
    Posso, Carmen Elisa
    Duque, Myriam Cristina
    MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2007, 102 (03): : 255 - 262
  • [33] SENSITIVE AND SPECIFIC COLORIMETRIC DOT ASSAY TO DETECT EASTERN EQUINE ENCEPHALOMYELITIS VIRAL-RNA IN MOSQUITOS (DIPTERA, CULICIDAE) AFTER POLYMERASE CHAIN-REACTION AMPLIFICATION
    ARMSTRONG, P
    BOROVSKY, D
    SHOPE, RE
    MORRIS, CD
    MITCHELL, CJ
    KARABATSOS, N
    KOMAR, N
    SPIELMAN, A
    JOURNAL OF MEDICAL ENTOMOLOGY, 1995, 32 (01) : 42 - 52
  • [34] A multiplex real-time polymerase chain reaction assay with two internal controls for the detection of Brucella species in tissues, blood, and feces from marine mammals
    Sidor, Inga F.
    Dunn, J. Lawrence
    Tsongalis, Gregory J.
    Carlson, Jolene
    Frasca, Salvatore, Jr.
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2013, 25 (01) : 72 - 81
  • [35] Descriptions of adults, immature stages and venom apparatus of two new species of Eudinostigma Tobias (Hymenoptera, Braconidae), hyperparasitoids of Phryxe caudata (Rondani) (Diptera, Tachinidae)
    Fischer, Maximilian
    Tormos, Josep
    Pardo, Xavier
    Asis, Josep Daniel
    Gayubo, Severiano F.
    Lopez, Emili
    Selfa, Jesus
    REVUE SUISSE DE ZOOLOGIE, 2006, 113 (04) : 829 - 839
  • [36] Real-time polymerase chain reaction testing for the detection of mycobacterium genavense and mycobacterium avium complex species in avian samples
    Tell, LA
    Leutenegger, CM
    Larsen, RS
    Agnew, DW
    Keener, L
    Needham, ML
    Rideout, BA
    AVIAN DISEASES, 2003, 47 (04) : 1406 - 1415
  • [37] Genetics and morphological differences of immature stages of two races of Rhagoletis conversa (Brethes) (Diptera: Tephritidae) associated to Solanum plants:: geographic distribution and possible sympatric origin of a new species
    Frías, DA
    REVISTA CHILENA DE HISTORIA NATURAL, 2001, 74 (01) : 73 - 90
  • [38] An ospA-polymerase chain reaction/restriction fragment length polymorphism-based method for sensitive detection and reliable differentiation of all European Borrelia burgdorferi sensu lato species and OspA types
    Michel, H
    Wilske, B
    Hettche, G
    Göttner, G
    Heimerl, C
    Reischl, U
    Schulte-Spechtel, U
    Fingerle, V
    MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 2004, 193 (04) : 219 - 226
  • [39] An ospA-polymerase chain reaction/restriction fragment length polymorphism-based method for sensitive detection and reliable differentiation of all European Borrelia burgdorferi sensu lato species and OspA types
    H. Michel
    B. Wilske
    G. Hettche
    G. Göttner
    C. Heimerl
    U. Reischl
    U. Schulte-Spechtel
    V. Fingerle
    Medical Microbiology and Immunology, 2004, 193 : 219 - 226
  • [40] Descriptions of adults, immature stages and venom apparatus of two new species of Dacnusini:: Chorebus pseudoasphodeli sp n., parasitic on Phytomyza chaerophili Kaltenbach and C pseudoasramenes sp n., parasitic on Cerodontha phragmitophila Hering (Hymenoptera: Braconidae: Alysiinae; Diptera: Agromyzidae)
    Tormos, J
    Pardo, X
    Jiménez, R
    Asís, JD
    Gayubo, SF
    EUROPEAN JOURNAL OF ENTOMOLOGY, 2003, 100 (03) : 393 - 400
1234