Species identification of the medicinal plant Tulipa edulis (Liliaceae) by DNA barcode marker

被引：17
作者：
Ma, Hong-liang [1 ,2 ]
Zhu, Zai-biao [1 ]
Zhang, Xiao-ming [1 ]
Miao, Yuan-yuan [1 ]
Guo, Qiao-sheng [1 ]
机构：
[1] Nanjing Agr Univ, Inst Chinese Med Mat, Nanjing 210095, Jiangsu, Peoples R China
[2] Guangzhou Baiyunshan Zhongyi Pharmaceut Co Ltd, Guangzhou 510530, Guangdong, Peoples R China
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2014年 / 55卷
基金：
中国国家自然科学基金;
关键词：
Tulipa edulis; DNA barcode; matK; rbcL; psbA-trnH; GENE;
D O I：
10.1016/j.bse.2014.03.038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Tulipa edulis (Liliaceae) is the botanical origin of the traditional Chinese medicine (TCM) "Guangcigu". Due to overexploitation that induced a decline in natural sources, many dried bulbs from other species of Tulipa have been used, adulterating the medicine in recent years. This practice may cause a series of inconsistent therapeutic effects and quality control problems in the herbal medicine industry. Hence, three DNA regions (matK, psbA-trnH and rbcL) were evaluated as barcodes for identifying T edulis and its adulterants. All candidate DNA barcodes were successfully amplified from leaf samples. Based on the sequence divergences, rbcL and psbA-trnH can assign T. edulis and its adulterants to the correct genus, while matK can accurately differentiate T edulis and its adulterants. Thus, at the DNA level, the matK intergenic region is a more suitable, accurate and applicable identification of T. edulis and its adulterants than rbcL and psbA-trnH. (C) 2014 Elsevier Ltd. All rights reserved.
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收藏
页码：362 / 368
页数：7
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