A gentle approach to investigate the influence of LRP-1 silencing on the migratory behavior of breast cancer cells by atomic force microscopy and dynamic cell studies

被引:11
作者
Berquand, Alexandre [1 ,2 ]
Meunier, Marie [3 ]
Thevenard-Devy, Jessica [3 ]
Ivaldi, Corinne [3 ]
Campion, Oceane [3 ]
Dedieu, Stephane [3 ]
Molinari, Michael [1 ,2 ,4 ]
Devy, Jerome [3 ]
机构
[1] Univ Reims, LRN, EA4682, F-51685 Reims, France
[2] Univ Reims, NanoMat Platform, F-51685 Reims, France
[3] Univ Reims, CNRS UMR 7369, Lab SiRMa, UFR Sci Exactes & Nat,Matrice Extracellulaire & D, Campus Moulin Housse,BP 1039, Reims, France
[4] Univ Bordeaux, INP Bordeaux, CNRS UMR 5248, Matrice Extracellulaire & Dynam Inst,CBMN, Pessac, France
关键词
LRP-1; Triple negative breast cancer; Atomic force microscopy; RECEPTOR-RELATED PROTEIN; ALPHA(2)-MACROGLOBULIN RECEPTOR; ELASTIC-MODULUS; EXPRESSION; MECHANICS; RHO; METASTASIS; STIFFNESS; INVASION; ADHESION;
D O I
10.1016/j.nano.2018.10.012
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The aim of the study was to get more insight into the role of LRP-1 in the mechanism of tumor progression in triple negative breast cancer. Atomic force microscopy, videomicroscopy, confocal microscopy and Rho-GTPAse activity assay were used on MDA-MB-231 and LRP-1-silenced cells. Silencing of LRP-1 in MDA-MB-231 cells was shown to led to a dramatic increase in the Young's modulus in parallel to a spectacular drop in membrane extension dynamics as well as a decrease in the cells migration abilities on both collagen I and fibronectin substrates. These results were perfectly correlated to a corresponding change in cell morphology and spreading capacity as well as in Rho-GTPases activity. By a multi-technique approach, it was demonstrated that LRP-1 played a crucial role in the migration of MDA-MB-231 cells by modulating the membrane extension dynamic. The originality of this AFM investigation lies in the non-invasive aspect of the measurements. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码:359 / 370
页数:12
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