Biocompatibility assessment of porous chitosan-Nafion and chitosan-PTFE composites in vivo

被引：10

作者：

Liu, Bo-Ji ^{[1
]}

Ma, Li-Nan ^{[2
]}

Su, Juan ^{[3
]}

Jing, Wei-Wei ^{[3
]}

Wei, Min-Jie ^{[4
]}

Sha, Xian-Zheng ^{[3
]}

机构：

[1] Sun Yat Sen Univ, Ctr Canc, Dept Radiat Oncol, State Key Lab Oncol South China, Guangzhou 510275, Guangdong, Peoples R China

[2] Patent Examinat Cooperat Ctr SIPO, Guangzhou, Guangdong, Peoples R China

[3] China Med Univ, Dept Biomed Engn, Shenyang, Peoples R China

[4] China Med Univ, Dept Pharmacol, Sch Pharmaceut Sci, Shenyang, Peoples R China

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2014年 / 102卷 / 06期

关键词：

chitosan; porous composite; biocompatibility; foreign body response; implantable sensor; TISSUE; MEMBRANE; COLLAGEN; CHITIN; VITRO;

D O I：

10.1002/jbm.a.34830

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Chitosan (CS) is widely used as a scaffold material in tissue engineering. The objective of this study was to test whether porous chitosan membrane (PCSM) coating for Nafion used in implantable sensor reduced fibrous capsule (FC) density and promoted superior vascularization compared with PCSM coating for polytetrafluoroethylene (PTFE). PCSM was fabricated with solvent casting/particulate leaching method using silica gel as porogen and characterized in vitro. Then, PCSM-Nafion and PCSM-PTFE composites were assembled with hydrated PCSM and implanted subcutaneously in rats. The histological analysis was performed in comparison with Nafion and PTFE. Implants were explanted 35, 65, and 100 days after the implantation. Histological assessments indicated that both composites achieved presumed effects of porous coatings on decreasing collagen deposition and promoting angiogenesis. PCSM-PTFE exerted higher collagen deposition by area ratio, both within and outside, compared with that of PCSM-Nafion. Angiogenesis within and outside the PCSM-Nafion both increased over time, but that of the PCSM-PTFE within decreased. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2055-2060, 2014.

引用

页码：2055 / 2060

页数：6

共 19 条

[1] Evaluation of the effect of the degree of acetylation on the inflammatory response to 3D porous chitosan scaffolds [J].

Barbosa, Judite N. ;

Amaral, Isabel F. ;

Aguas, Artur P. ;

Barbosa, Mario A. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2010, 93A (01) :20-28

[2] NEOVASCULARIZATION OF SYNTHETIC MEMBRANES DIRECTED BY MEMBRANE MICROARCHITECTURE [J].

BRAUKER, JH ;

CARRBRENDEL, VE ;

MARTINSON, LA ;

CRUDELE, J ;

JOHNSTON, WD ;

JOHNSON, RC .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1995, 29 (12) :1517-1524

[3] Nitric oxide monitoring in brain extracellular fluid: characterisation of Nafion®-modified Pt electrodes in vitro and in vivo [J].

Brown, Finbar O. ;

Finnerty, Niall J. ;

Lowry, John P. .

ANALYST, 2009, 134 (10) :2012-2020

[4] Study of the effects of tissue reactions on the function of implanted glucose sensors [J].

Dungel, Paul ;

Long, Nathan ;

Yu, Bazhang ;

Moussy, Yvonne ;

Moussy, Francis .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 85A (03) :699-706

[5] Collagen - biomaterial for drug delivery [J].

Friess, W .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 1998, 45 (02) :113-136

[6] Chitosan-coated stainless steel screws for fixation in contaminated fractures [J].

Greene, Alex H. ;

Bumgardner, Joel D. ;

Yang, Yunzhi ;

Moseley, Jon ;

Haggard, Warren O. .

CLINICAL ORTHOPAEDICS AND RELATED RESEARCH, 2008, 466 (07) :1699-1704

[7] A new and evolving paradigm for biocompatibitity [J].

Hilborn, Jons ;

Bjursten, Lars M. .

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2007, 1 (02) :110-119

[8] A novel porous collagen scaffold around an implantable biosensor for improving biocompatibility.: I.: In vitro/in vivo stability of the scaffold and in vitro sensitivity of the glucose sensor with scaffold [J].

Ju, Young Min ;

Yu, Bazhang ;

Koob, Thomas J. ;

Moussy, Yvonne ;

Moussy, Francis .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 87A (01) :136-146

[9] A novel porous collagen scaffold around an implantable biosensor for improving biocompatibility. II. Long-term in vitro/in vivo sensitivity characteristics of sensors with NDGA- or GA-crosslinked collagen scaffolds [J].

Ju, Young Min ;

Yu, Bazhang ;

West, Leigh ;

Moussy, Yvonne ;

Moussy, Francis .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2010, 92A (02) :650-658

[10] A biodegradable vascularizing membrane: A feasibility study [J].

Kaushiva, Anchal ;

Turzhitsky, Vladimir M. ;

Backman, Vadim ;

Ameer, Guillermo A. .

ACTA BIOMATERIALIA, 2007, 3 (05) :631-642

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