Cross-species application of sex linked markers in H-salicifolia and H-tibetana

被引：6

作者：

Chawla, Aseem ^{[1
]}

Kant, Anil ^{[1
]}

Stobdan, Tsering ^{[2
]}

Srivastava, Ravi B. ^{[2
]}

Chauhan, R. S. ^{[1
]}

机构：

[1] Jaypee Univ Informat Technol, Dept Biotechnol & Bioinformat, Waknaghat 173215, Solan, India

[2] Def R&D Org, Def Inst High Altitude Res, Leh Ladakh 194101, India

来源：

SCIENTIA HORTICULTURAE | 2014年 / 170卷

关键词：

SCAR marker; Seabuckthorn; Sex determination; HIPPOPHAE-SALICIFOLIA;

D O I：

10.1016/j.scienta.2014.03.013

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Early sex determination of dioecious plants is essential for successful genetic improvement and commercial cultivation. Male and female seabuckthorn plants are morphologically similar and cannot be distinguished prior to 3-4 years of growth, i.e., at the time of flowering. India has three species of seabuckthorn namely Hippophae rhamnoides, Hippophae salicifolia and Hippophae tibetana. Applicability of sex linked SCAR markers HrX1 and HrX2 developed for H. rhamnoides were tested in H. salicifolia and H. tibetana using H. rhamnoides as control. HrX1 produced female specific amplification in both H. salicifolia and H. tibetana while HrX2 did not amplify except for H. rhamnoides. Sequence based analysis of amplified SCAR marker products HrX1, HsX1 and HtX1 has shown homology with known plant acyl CoA ligases. (c) 2014 Elsevier B.V. All rights reserved.

引用

页码：281 / 283

页数：3