Lactobacillus plantarum vaccine vector expressing hemagglutinin provides protection against H9N2 challenge infection

被引：51
作者：
Shi, Shao-Hua [1 ]
Yang, Wen-Tao [1 ]
Yang, Gui-Lian [1 ]
Zhang, Xu-Ke [2 ]
Liu, Yu-Ying [1 ]
Zhang, Li-Jiao [1 ]
Ye, Li-Ping [1 ]
Hu, Jing-Tao [1 ]
Xing, Xin [1 ]
Qi, Chong [3 ]
Li, Yu [1 ]
Wang, Chun-Feng [1 ]
机构：
[1] Jilin Agr Univ, Coll Anim Sci & Technol, Jilin Prov Engn Res Ctr Anim Probiot, Engn Res Ctr,Chinese Minist Educ Edible & Med Fun, Changchun 130118, Peoples R China
[2] Pulike Biol Engn INC, Luoyang 471000, Peoples R China
[3] Jilin Univ, Bethune Hosp Acad Translat Med 1, Changchun 130021, Peoples R China
来源：
VIRUS RESEARCH | 2016年 / 211卷
基金：
中国国家自然科学基金;
关键词：
Hemagglutinin; Dendritic cell-targeting peptide; Lactobacillus plantarum; H9N2 influenza virus; Mucosal vaccination; PATHOGENIC AVIAN INFLUENZA; IMMUNE-RESPONSES; DENDRITIC CELLS; MUCOSAL VACCINE; VIRUS; MICE; ANTIGEN; CHINA; IMMUNIZATION; ANTIBODIES;
D O I：
10.1016/j.virusres.2015.09.005
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Hemagglutinin (HA) has been demonstrated as an effective candidate vaccine antigen against AIVs. Dendritic cell-targeting peptide (DCpep) can enhance the robustness of immune responses. The purpose of this study was to evaluate whether DCpep could enhance the immune response against H9N2 AIV when utilizing Lactobacillus plantarum NC8 (NC8) to present HA-DCpep in mouse and chicken models. To accomplish this, a mucosal vaccine of a recombinant NC8 strain expressing HA and DCpep that was constructed in a previous study was employed. Orally administered NC8-pSIP409-HA-DCpep elicited high serum titers of hemagglutination-inhibition (HI) antibodies in mice and also induced robust T cell immune responses in both mouse and chicken models. Orally administered NC8-pSIP409-HA-DCpep elicited high serum titers of hemagglutination-inhibition (HI) antibodies in mice and also induced robust T cell immune responses in both mouse and chicken models. These results revealed that recombinant L plantarum NC8-pSIP409-HA-DCpep is an effective vaccine candidate against H9N2 AIVs. (C) 2015 Elsevier B.V. All rights reserved.
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收藏
页码：46 / 57
页数：12
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