Identification of the Enterococcus faecalis tyrosine decarboxylase operon involved in tyramine production

被引:113
作者
Connil, N
Le Breton, Y
Dousset, X
Auffray, Y
Rincé, A
Prévost, H
机构
[1] ENITIAA, LMAI, F-44322 Nantes 03, France
[2] Univ Caen, INRA, IRBA, EA956,Lab Microbiol Environm, F-14032 Caen, France
关键词
D O I
10.1128/AEM.68.7.3537-3544.2002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Screening of a library of Enterococcus faecalis insertional mutants allowed isolation of a mutant affected in tyramine production. The growth of this mutant was similar to that of the wild-type E. faecalis JH2-2 strain in Maijala broth, whereas high-performance liquid chromatography analyses showed that tyramine production, which reached 1,000 mug ml(-1) for the wild-type strain, was completely abolished. Genetic analysis of the insertion locus revealed a gene encoding a decarboxylase with similarity to eukaryotic tyrosine decarboxylases. Sequence analysis revealed a pyridoxal phosphate binding site, indicating that this enzyme belongs to the family of amino acid decarboxylases using this cofactor. Reverse transcription-PCR analyses demonstrated that the gene (tdc) encoding the putative tyrosine decarboxylase of E. faecalis JH2-2 is cotranscribed with the downstream gene encoding a putative tyro sine-tyramine antiporter and with the upstream tyrosyl-tRNA synthetase gene. This study is the first description of a tyrosine decarboxylase gene in prokaryotes.
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页码:3537 / 3544
页数:8
相关论文
共 57 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]   UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM [J].
AMES, GF .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1964, 104 (01) :1-&
[3]   THE TYROSYL-TRANSFER RNA-SYNTHETASE FROM ESCHERICHIA-COLI - COMPLETE NUCLEOTIDE-SEQUENCE OF THE STRUCTURAL GENE [J].
BARKER, DG ;
BRUTON, CJ ;
WINTER, G .
FEBS LETTERS, 1982, 150 (02) :419-423
[4]   PURIFICATION AND CHARACTERIZATION OF TYROSINE DECARBOXYLASE AND AROMATIC-L-AMINO-ACID DECARBOXYLASE [J].
BORRESEN, T ;
KLAUSEN, NK ;
LARSEN, LM ;
SORENSEN, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 993 (01) :108-115
[5]   Improved screening procedure for biogenic amine production by lactic acid bacteria [J].
Bover-Cid, S ;
Holzapfel, WH .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 53 (01) :33-41
[6]   FORMATION OF AROMATIC AMINO ACID POOLS IN ESCHERICHIA-COLI K-12 [J].
BROWN, KD .
JOURNAL OF BACTERIOLOGY, 1970, 104 (01) :177-&
[7]   Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].
Bult, CJ ;
White, O ;
Olsen, GJ ;
Zhou, LX ;
Fleischmann, RD ;
Sutton, GG ;
Blake, JA ;
FitzGerald, LM ;
Clayton, RA ;
Gocayne, JD ;
Kerlavage, AR ;
Dougherty, BA ;
Tomb, JF ;
Adams, MD ;
Reich, CI ;
Overbeek, R ;
Kirkness, EF ;
Weinstock, KG ;
Merrick, JM ;
Glodek, A ;
Scott, JL ;
Geoghagen, NSM ;
Weidman, JF ;
Fuhrmann, JL ;
Nguyen, D ;
Utterback, TR ;
Kelley, JM ;
Peterson, JD ;
Sadow, PW ;
Hanna, MC ;
Cotton, MD ;
Roberts, KM ;
Hurst, MA ;
Kaine, BP ;
Borodovsky, M ;
Klenk, HP ;
Fraser, CM ;
Smith, HO ;
Woese, CR ;
Venter, JC .
SCIENCE, 1996, 273 (5278) :1058-1073
[8]   Escherichia coli acid resistance:: cAMP receptor protein and a 20 bp cis-acting sequence control pH and stationary phase expression of the gadA and gadBC glutamate decarboxylase genes [J].
Castanie-Cornet, MP ;
Foster, JW .
MICROBIOLOGY-UK, 2001, 147 :709-715
[9]  
Coton E, 1998, AM J ENOL VITICULT, V49, P199
[10]   A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid [J].
Cotter, PD ;
Gahan, CGM ;
Hill, C .
MOLECULAR MICROBIOLOGY, 2001, 40 (02) :465-475