Effect of Curcumin and Pirfenidone on Toxicokinetics of Paraquat in Rat by UPLC-MS/MS

被引：40

作者：

Wang, Shuanghu ^{[1
]}

Lin, Zixia ^{[2
]}

Su, Ke ^{[2
]}

Zhang, Jing ^{[2
]}

Zhang, Lijing ^{[2
]}

Gao, Zhimou ^{[3
]}

Wang, Zhiyi ^{[4
,5
]}

Ma, Jianshe ^{[2
]}

Wang, Xianqin ^{[2
]}

机构：

[1] Peoples Hosp Lishui, Lab Clin Pharm, Lishui 323000, Peoples R China

[2] Wenzhou Med Univ, Analyt & Testing Ctr, Wenzhou 325035, Peoples R China

[3] Anhui Agr Univ, Coll Plant Protect, Hefei 230036, Anhui, Peoples R China

[4] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Peoples R China

[5] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Peoples R China

来源：

ACTA CHROMATOGRAPHICA | 2018年 / 30卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Paraquat; UPLC-MS/MS; toxicokinetics; rat; curcumin; pirfenidone; PERFORMANCE LIQUID-CHROMATOGRAPHY; SUPPORT VECTOR MACHINE; METABOLIC-CHANGES; POISONED PATIENTS; PLASMA; MODEL;

D O I：

10.1556/1326.2017.00175

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The rats were randomly divided into paraquat group, curcumin treatment group, and pirfenidone treatment group. The concentration of paraquat in rat plasma was determined by an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method over the range of 10-2000 ng mL(-1). Chromatographic separation was achieved on a BEH HILIC (2.1 mm x 100 mm, 1.7 mu m) column. The mobile phase was consisted of acetonitrile and 10 mm ammonium formate buffer (containing 0.1% formic acid) with gradient elution pumped at a flow rate of 0.4 mL min(-1). Protein precipitation with acetonitrile was used as sample preparation. Compared with the paraquat group, there is statistical toxicokinetic difference for curcumin treatment group and pirfenidone treatment group, AUC((0 -t)) decreased (P < 0.05), clearance (CL) increased (P < 0.05) for curcumin or pirfenidone treatment group, and C-max decreased (P < 0.05) for curcumin treatment group. The results showed that treatment by curcumin and pirfenidone could relieve acute paraquat poisoning in rats.

引用

页码：26 / 30

页数：5

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