Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test

被引：12

作者：

Carvalho do Espirito-Santo, Maria Cristina ^{[1
]}

Pinto, Pedro Luiz ^{[2
]}

Gargioni, Cybele ^{[3
]}

Alvarado-Mora, Monica Viviana ^{[4
]}

Pagliusi Castilho, Vera Lucia ^{[5
]}

Rebello Pinho, Joao Ranato ^{[6
]}

de Albuquerque Luna, Expedito Jose ^{[7
]}

Borges Gryschek, Ronaldo Cesar ^{[8
]}

机构：

[1] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Sao Paulo, Brazil

[2] Adolfo Lutz Inst, Dept Enteroparasites Parasitol, Sao Paulo, Brazil

[3] Adolfo Lutz Inst, Dept Enteroparasites Parasitol & Mycol, Sao Paulo, Brazil

[4] Univ Sao Paulo, Sch Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil

[5] Univ Sao Paulo, Fac Med, Cent Lab, Lab Clin Parasitol,Hosiital Clin, Sao Paulo, Brazil

[6] Univ Sao Paulo, Fac Med, Dept Gastroenterol, Lab Trop Gastroenterol & Hepatol, Sao Paulo, Brazil

[7] Univ Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, Brazil

[8] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, Sao Paulo, Brazil

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2014年 / 90卷 / 06期

关键词：

LOW-TRANSMISSION AREAS; HUMAN INFECTIONS; DIAGNOSIS; ANTIGENS; CHINA; EGGS; IMMUNODIAGNOSIS; PREVALENCE; VENEZUELA; BRAZIL;

D O I：

10.4269/ajtmh.13-0746

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.

引用

页码：1146 / 1152

页数：7

共 14 条

[1] Detection of low-intensity Schistosoma mansoni infection by Percoll sedimentation and real-time PCR techniques in a low-endemicity Egyptian setting
Allam, Amal F.
Farag, Hoda F.
Zaki, Adel
Kader, Ola A.
Abdul-Ghani, Rashad
Shehab, Amel Y.
TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2015, 20 (05) : 658 - 664
[2] Sensitivity of the Point-of-Care Circulating Cathodic Antigen Urine Cassette Test for Diagnosis of Schistosoma mansoni in Low-Endemicity Settings in Cote d'Ivoire
Assare, Rufin K.
Tra, Mathieu B. I.
Ouattara, Mamadou
Hurlimann, Eveline
Coulibaly, Jean T.
N'Goran, Eliezer K.
Utzinger, Jurg
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2018, 99 (06) : 1567 - 1572
[3] A new rapid diagnostic test for detection of anti-Schistosoma mansoni and anti-Schistosoma haematobium antibodies
Coulibaly, Jean T.
N'Goran, Eliezer K.
Utzinger, Juerg
Doenhoff, Michael J.
Dawson, Emily M.
PARASITES & VECTORS, 2013, 6
[4] Assessing the marginal error in diagnosis and cure of Schistosoma mansoni in areas of low endemicity using Percoll and PCR techniques
Allam, Amal Farahat
Kader, Ola
Zaki, Adel
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Farag, Hoda Fahmy
TROPICAL MEDICINE & INTERNATIONAL HEALTH, 2009, 14 (03) : 316 - 321
[5] Newly Established Monoclonal Antibody Diagnostic Assays for Schistosoma mansoni Direct Detection in Areas of Low Endemicity
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Zech Coelho, Paulo Marcos
Taboada, Diana
Alves de Mattos, Ana Carolina
Davis, Ruth
Harn, Donald A.
PLOS ONE, 2014, 9 (01):
[6] Detection of IgG antibodies against Trypanosoma vivax in cattle by indirect immunofluorescence test
Guerra, Neurisvan R.
Monteiro, Maria F. M.
Sandes, Hevila M. M.
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Ramos, Carlos A. N.
de Assis Santana, Vania Lucia
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Alves, Leucio Camara
PESQUISA VETERINARIA BRASILEIRA, 2013, 33 (12): : 1423 - 1426
[7] A new rapid diagnostic test for detection of anti-Schistosoma mansoni and anti-Schistosoma haematobium antibodies
Jean T Coulibaly
Eliézer K N’Goran
Jürg Utzinger
Michael J Doenhoff
Emily M Dawson
Parasites & Vectors, 6
[8] Assessment of the accuracy of 11 different diagnostic tests for the detection of Schistosomiasis mansoni in individuals from a Brazilian area of low endemicity using latent class analysis
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FRONTIERS IN MICROBIOLOGY, 2022, 13
[9] Evaluating a point-of-care circulating cathodic antigen test (POC-CCA) to detect ⁢Schistosoma mansoni⁢ infections in a low endemic area in north-eastern Brazil
Moraes Bezerra, Fernando Schemelzer
Freitas Leal, Joames Kauffimann
Sousa, Mariana Silva
Cunha Pinheiro, Marta Cristhiany
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ACTA TROPICA, 2018, 182 : 264 - 270
[10] Performance of an Ultra-Sensitive Assay Targeting the Circulating Anodic Antigen (CAA) for Detection of Schistosoma mansoni Infection in a Low Endemic Area in Brazil
Sousa, Mariana Silva
van Dam, Govert J.
Cunha Pinheiro, Marta Cristhiany
de Dood, Claudia J.
Peralta, Jose Mauro
Saramago Peralta, Regina Helena
Daher, Elizabeth de Francesco
Corstjens, Paul L. A. M.
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FRONTIERS IN IMMUNOLOGY, 2019, 10

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