Advances in the development of molecular genetic tools for Mycobacterium tuberculosis

被引：13

作者：

Chhotaray, Chiranjibi ^{[1
,2
]}

Tan, Yaoju ^{[3
]}

Mugweru, Julius ^{[1
,2
,4
]}

Islam, Md Mahmudul ^{[1
,2
]}

Hameed, H. M. Adnan ^{[1
,2
]}

Wang, Shuai ^{[1
,2
]}

Lu, Zhili ^{[1
]}

Wang, Changwei ^{[1
]}

Li, Xinjie ^{[3
]}

Tan, Shouyong ^{[3
]}

Liu, Jianxiong ^{[3
]}

Zhang, Tianyu ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, State Key Lab Resp Dis, Guangzhou 510530, Guangdong, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Guangzhou Chest Hosp, Dept Clin Lab, State Key Lab Resp Dis, Guangzhou 510095, Guangdong, Peoples R China

[4] Univ Embu, Dept Biol Sci, POB 6-60100, Embu, Kenya

来源：

JOURNAL OF GENETICS AND GENOMICS | 2018年 / 45卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Mycobacterium tuberculosis; Molecular genetic tools; Gram-positive; Recombineering; Drug resistance; SIGNATURE-TAGGED MUTAGENESIS; SITE-SPECIFIC INTEGRATION; MULTIDRUG-RESISTANT TUBERCULOSIS; LUCIFERASE REPORTER PHAGES; BACTERIOPHAGE-BASED METHOD; ESCHERICHIA-COLI; DRUG-RESISTANT; SINGLE-MOLECULE; PHENOTYPIC CHARACTERIZATION; CONDITIONAL EXPRESSION;

D O I：

10.1016/j.jgg.2018.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mycobacterium tuberculosis, a Gram-positive bacterium of great clinical relevance, is a lethal pathogen owing to its complex physiological characteristics and development of drug resistance. Several molecular genetic tools have been developed in the past few decades to study this microorganism. These tools have been instrumental in understanding how M. tuberculosis became a successful pathogen. Advanced molecular genetic tools have played a significant role in exploring the complex pathways involved in M. tuberculosis pathogenesis. Here, we review various molecular genetic tools used in the study of M. tuberculosis. Further, we discuss the applications of clustered regularly interspaced short palindromic repeat interference (CRISPRi), a novel technology recently applied in M. tuberculosis research to study target gene functions. Finally, prospective outcomes of the applications of molecular techniques in the field of M. tuberculosis genetic research are also discussed. Copyright (C) 2018, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.

引用

页码：281 / 297

页数：17

共 267 条

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