Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action

被引：97

作者：

Zhang, QX

Pilquil, CS

Dewald, J

Berthiaume, LG

Brindley, DN

机构：

[1] Univ Alberta, Dept Biochem, Signal Transduct Labs, Lipid & Lipoprot Res Grp, Edmonton, AB T6G 2S2, Canada

[2] Univ Alberta, Dept Cell Biol, Lipid & Lipoprot Res Grp, Edmonton, AB T6G 2S2, Canada

来源：

BIOCHEMICAL JOURNAL | 2000年 / 345卷

关键词：

ecto-enzyme; glycosylation; lysophosphatidate; phosphatidate phosphohydrolase;

D O I：

10.1042/0264-6021:3450181

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipid phosphate phosphatase-1 (LPP-1) dephosphorylates exogenous lysophosphatidate and thereby regulates the activation of lysophosphatidate receptors and cell division. Mutation of seven amino acids in three conserved domains of mouse LPP-1 abolished its activity. A glycosylation site was demonstrated between conserved Domains 1 and 2. LPP-1 is expressed in the plasma membrane, and the present results demonstrate the active site to be located on the outer surface.

引用

收藏

页码：181 / 184

页数：4

相关论文

共 24 条

[1] Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid [J].

An, SZ ;

Bleu, T ;

Hallmark, OG ;

Goetzl, EJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (14) :7906-7910

[2]

ANDERSSON S, 1989, J BIOL CHEM, V264, P8222

[3] The Dri 42 gene, whose expression is up-regulated during epithelial differentiation, encodes a novel endoplasmic reticulum resident transmembrane protein [J].

Barila, D ;

Plateroti, M ;

Nobili, F ;

Muda, AO ;

Xie, YH ;

Morimoto, T ;

Perozzi, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (47) :29928-29936

[4] Mammalian lipid phosphate phosphohydrolases [J].

Brindley, DN ;

Waggoner, DW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (38) :24281-24284

[5] A single receptor encoded by vzg-1/lpA1/edg-2 couples to G proteins and mediates multiple cellular responses to lysophosphatidic acid [J].

Fukushima, N ;

Kimura, Y ;

Chun, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) :6151-6156

[6] Molecular cloning of a high-affinity receptor for the growth factor-like lipid mediator lysophosphatidic acid from Xenopus oocytes [J].

Guo, Z ;

Liliom, K ;

Fischer, DJ ;

Bathurst, IC ;

Tomei, LD ;

Kiefer, MC ;

Tigyi, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14367-14372

[7] A new model for the membrane topology of glucose-6-phosphatase: The enzyme involved in von Gierke disease [J].

Hemrika, W ;

Wever, R .

FEBS LETTERS, 1997, 409 (03) :317-319

[8] From phosphatases to vanadium peroxidases: A similar architecture of the active site [J].

Hemrika, W ;

Renirie, R ;

Dekker, HL ;

Barnett, P ;

Wever, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2145-2149

[9] SITE-DIRECTED MUTAGENESIS BY OVERLAP EXTENSION USING THE POLYMERASE CHAIN-REACTION [J].

HO, SN ;

HUNT, HD ;

HORTON, RM ;

PULLEN, JK ;

PEASE, LR .

GENE, 1989, 77 (01) :51-59

[10] Identification of a novel human phosphatidic acid phosphatase type 2 isoform [J].

Hooks, SB ;

Ragan, SP ;

Lynch, KR .

FEBS LETTERS, 1998, 427 (02) :188-192