Accurate base composition of double-strand DNA by mass spectrometry

被引：28

作者：

Aaserud, DJ ^{[1
]}

Kelleher, NL ^{[1
]}

Little, DP ^{[1
]}

McLafferty, FW ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT CHEM,BAKER LAB CHEM,ITHACA,NY 14853

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1996年 / 7卷 / 12期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S1044-0305(96)00194-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An accurate molecular weight (M(r)) assignment for a double-strand (ds) DNA determines or greatly restricts the possible number of each of its four bases, while the compositions for its two single-strand (ss) components can also be derived from their M(r) values. For a ds 64-mer (39 kDa), the ss-MI values (+/-0.5 Da) of its high-resolution mass spectrum from an electrospray ionization/Fourier transform instrument yield only the correct ds- and ss-base compositions. Literature mass spectra of lower mass accuracy show that such data can also restrict their possible composition assignments, with further discrimination using the abundance vs. base composition of small fragment ions from the dissociation of the ss molecular ions. (C) 1996 American Society for Mass Spectrometry.

引用

页码：1266 / 1269

页数：4

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