Ethylammonium nitrate: a protein crystallization reagent

被引：87

作者：

Garlitz, JA ^{[1
]}

Summers, CA ^{[1
]}

Flowers, RA ^{[1
]}

Borgstahl, GEO ^{[1
]}

机构：

[1] Univ Toledo, Dept Chem, Toledo, OH 43606 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1999年 / 55卷

关键词：

D O I：

10.1107/S0907444999011774

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylammonium nitrate (EAN) is a liquid organic salt that has many potential applications in protein chemistry. Because this solvent has hydrophobic and ionic character as well as the ability to hydrogen bond, it is especially well suited for broad use in protein crystallography. For example, EAN may be used as an additive, a detergent, a precipitating agent or to deliver ligands into protein crystals. A discussion of the crystallization of lysozyme using EAN as a precipitating agent is given here.

引用

页码：2037 / 2038

页数：2

共 5 条

[1]

Brunger A. T., 1992, X PLOR VERSION 3 1 S

[2] MICELLE FORMATION IN ETHYLAMMONIUM NITRATE, A LOW-MELTING FUSED SALT [J].