LIPOSOMES AS DRUG CARRIERS AND THEIR CHARACTERIZATION USING DIFFERENT ANALYTICAL METHODS

The physicochemical properties of liposomes are significantly affected by the composition of phospholipid bilayer; differences in composition allow the use of liposomes for analytical purposes and for therapeutic purposes. One of the most used components of phospholipid bilayer is cholesterol. Its concentration plays a significant role in the behaviour of liposomes. This study points to changes in the properties of liposomes and its influence on encapsulated doxorubicin according to the content of cholesterol in the phospholipid bilayer. The influence of SDS addition to liposomal variants was also evaluated. Three variants of liposomes differing in various concentrations of cholesterol were assessed. Firstly, the toxicity of all types of liposomal doxorubicin was evaluated and it was found that the content of cholesterol increases the IC50 values of encapsulated doxorubicin in liposome. The highest concentration of cholesterol in liposome increased the IC50 value even four times compared to liposomes without cholesterol. Secondly, the new approach to compare the influence of different variants of liposomes on detection of carried doxorubicin was used using the electrochemical detection with construction of differential hydrodynamic voltammograms.