Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils

被引：34

作者：

Mumaw, Jennifer L. ^{[1
]}

Schmiedt, Chad W. ^{[2
]}

Breidling, Sarah ^{[1
]}

Sigmund, Alex ^{[1
]}

Norton, Natalie A. ^{[1
]}

Thoreson, Merrilee ^{[1
]}

Peroni, John F. ^{[1
]}

Hurley, David J. ^{[1
]}

机构：

[1] Univ Georgia, Coll Vet Med, Dept Large Anim Med, Athens, GA 30602 USA

[2] Univ Georgia, Coll Vet Med, Dept Small Anim Med & Surg, Athens, GA 30602 USA

来源：

RESEARCH IN VETERINARY SCIENCE | 2015年 / 103卷

关键词：

Feline; Neutrophils; Mesenchymal stem cell; Reactive oxygen species; STROMAL CELLS; ISCHEMIA-REPERFUSION; IN-VITRO; THERAPY; KIDNEY; SAFETY; MODEL; DIFFERENTIATION; INTERLEUKIN-6; INDUCTION;

D O I：

10.1016/j.rvsc.2015.09.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Feline bone marrow-derived MSCs (BMMSCs), adipose-derived MSCs (AMSCs) and fibroblasts (FBs) were isolated and cultured. Tri-lineage differentiation assays and flow cytometry were used to characterize MSCs. Neutrophils (NPs) were isolated from whole blood and the NPs production of reactive oxygen reactive oxygen species (ROS) was measured. NPs were cultured alone, with MSC culture supernatant (SN), BMMSCs or AMSCs. NPs incubated with BMMSCs had significantly lower ROS production than NPs incubated with AMSCs (p = 0.0006) or FB (p <0.0001); NPs ROS production significantly decreased with increasing BMMSC cell number (p = 0.0023) and significantly increased with NPs were incubated with FB compared to BMMSC (p = 0.0003). Both BMMSC SN and AMSC SN had statistically significantly lower ROS production than FB SN when incubated with NPs (both p < 0.0001). ROS production was significantly reduced with increased fractions of SN from BMMSCs (p = 0.0467) and AMSCs (p = 0.0017). (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：60 / 69

页数：10

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