Determination of Moclobemide and its Metabolites in Human Plasma by SPE-HPLC-UV: Evaluation of Critical Experimental Conditions and QSRR Study

被引：2
作者：
Ignjatovic, Anita Rakic [1 ]
Nikolic, Katarina [2 ]
Miljkovic, Branislava [3 ]
Pokrajac, Milena [3 ]
Agbaba, Danica [2 ]
Prostran, Milica [4 ]
机构：
[1] Med & Med Devices Agcy Serbia, Belgrade 11152, Serbia
[2] Univ Belgrade, Fac Pharm, Inst Pharmaceut Chem & Drug Anal, Belgrade, Serbia
[3] Fac Pharm, Dept Pharmacokinet, Belgrade, Serbia
[4] Univ Belgrade, Sch Med, Dept Pharmacol Clin Pharmacol & Toxicol, Belgrade, Serbia
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2009年 / 32卷 / 14期
关键词：
HPLC; Metabolites; Moclobemide; Plasma; QSRR model; SPE; PERFORMANCE LIQUID-CHROMATOGRAPHY; STATIONARY PHASES; INHIBITOR MOCLOBEMIDE;
D O I：
10.1080/10826070903126963
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A SPE-HPLC-UV method for determination of moclobemide and its major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma had been developed previously and its selectivity was evaluated against the most frequently coadministered drugs. The objective of the present work was to develop a quantitative structure retention relationship (QSRR) model capable of providing good predictions of chromatographic behaviour of other related potentially interfering drugs, based on the previously generated data, in order to further improve the clinical applicability of the existing method. Moreover, the most critical factors affecting SPE and chromatographic separations of moclobemide and its metabolites were evaluated and discussed, taking into account molecular properties of the analyzed compounds.
引用
收藏
页码：2080 / 2096
页数：17
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