MyoD-induced circular RNA CDR1as promotes myogenic differentiation of skeletal muscle satellite cells

被引：76

作者：

Li, Li ^{[1
]}

Chen, Yuan ^{[1
]}

Nie, Lu ^{[1
]}

Ding, Xue ^{[1
]}

Zhang, Xiao ^{[1
]}

Zhao, Wei ^{[1
]}

Xu, Xiaoli ^{[1
]}

Kyei, Bismark ^{[1
]}

Dai, Dinghui ^{[1
]}

Zhan, Siyuan ^{[1
]}

Guo, Jiazhong ^{[1
]}

Zhong, Tao ^{[1
]}

Wang, Linjie ^{[1
]}

Zhang, Hongping ^{[1
]}

机构：

[1] Sichuan Agr Univ, Coll Anim Sci & Technol, 211 Huimin Rd, Chengdu 611130, Sichuan, Peoples R China

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS | 2019年 / 1862卷 / 08期

基金：

中国国家自然科学基金;

关键词：

CDR1; as; Myogenesis; Myogenic differentiation protein 1; Insulin like growth factor 1 receptor; microRNA; 7; Skeletal muscle satellite cell; GENE REGULATORY NETWORKS; LONG NONCODING RNA; EXPRESSION; MICRORNA; BIOGENESIS; PREDICTION; PROFILES; RECEPTOR; DATABASE; MIR-7;

D O I：

10.1016/j.bbagrm.2019.07.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Many protein coding and non-coding genes interplay in governing skeletal muscle formation. Nevertheless, comparing with the linear transcripts, functions of covalently closed circular RNAs (circRNAs), the new frontier of regulatory non-coding RNA (ncRNAs) molecules, remain largely unknown. Here, we identify CDR1as (antisense to the cerebellar degeneration-related protein 1 transcript, also termed as ciRS-7), a well-known cancer and neuron circRNA, plays a significant role in virtually controlling muscle differentiation. CDR1as is highly expressed in muscles of the mid-embryonic goat foetus, and activated at the initiation of myogenic differentiation in vitro. MyoD (myogenic differentiation protein 1), a driven transcription factor for myogenesis, promotes CDR1as by binding on its 5' flank region (- 646 to -634 bp, neighbouring the predicted transcription start site at - 580 bp). Overexpression or knockdown of CDR1as dramatically induces or impedes muscle differentiation program, respectively. By competitively binding to miR-7 (microRNA 7), CDR1as relieves the downregulation of IGF1R (insulin like growth factor 1 receptor) caused by miR-7 and consequently activates muscle differentiation. These results unveil that CDR1as plays critical roles in myogenic differentiation, which extends the versatile functions of CDR1as in mammal development and disease.

引用

页码：807 / 821

页数：15

共 72 条

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