On-Chip Fluorescence-Activated Cell Sorting by an Integrated Miniaturized Ultrasonic Transducer

被引:58
作者
Johansson, Linda [1 ]
Nikolajeff, Fredrik [1 ]
Johansson, Stefan [1 ]
Thorslund, Sara [1 ]
机构
[1] Uppsala Univ, Angstrom Lab, Dept Engn Sci, SE-75121 Uppsala, Sweden
关键词
STANDING-WAVE; MICROFLUIDIC CHIP; SINGLE CELLS; PARTICLE; SEPARATION; FLOW; SORTER; MANIPULATION; DEVICES; SYSTEM;
D O I
10.1021/ac802681r
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An acoustic microfluidic system for miniaturized fluorescence-activated cell sorting (mu FACS) is presented. By excitation of a miniaturized piezoelectric transducer at 10 MHz in the microfluidic channel bottom, an acoustic standing wave is formed in the channel. The acoustic radiation force acting on a density interface causes fluidic movement, and the particles or cells on either side of the fluid interface are displaced in a direction perpendicular to the standing wave direction. The small size of the transducer enables individual manipulation of cells passing the transducer surface. At constant transducer activation the system was shown to accomplish up to 700 mu m sideways displacement of 10 mu m beads in a 1 mm wide channel. This is much larger than if utilizing the acoustic radiation force acting directly on particles, where the limitation in maximum displacement is between a node and an antinode which at 10 MHz is 35 mu m. In the automatic sorting setup, the system was demonstrated to successfully sort single cells of E-GFP expressing beta-cells.
引用
收藏
页码:5188 / 5196
页数:9
相关论文
共 38 条
[1]   Molecular adhesion development in a neural cell monolayer forming in an ultrasound trap [J].
Bazou, D ;
Foster, GA ;
Ralphs, JR ;
Coakley, WT .
MOLECULAR MEMBRANE BIOLOGY, 2005, 22 (03) :229-240
[2]  
Bruus H., 2008, THEORETICAL MICROFLU
[3]   Optical micromanipulation [J].
Dholakia, Kishan ;
Reece, Peter ;
Gu, Min .
CHEMICAL SOCIETY REVIEWS, 2008, 37 (01) :42-55
[4]   An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles [J].
Dittrich, PS ;
Schwille, P .
ANALYTICAL CHEMISTRY, 2003, 75 (21) :5767-5774
[5]  
EVANDER M, 2006, MUETAS C P, P1055
[6]   Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays [J].
Evander, Mikael ;
Johansson, Linda ;
Lilliehorn, Tobias ;
Piskur, Jure ;
Lindvall, Magnus ;
Johansson, Stefan ;
Almqvist, Monica ;
Laurell, Thomas ;
Nilsson, Johan .
ANALYTICAL CHEMISTRY, 2007, 79 (07) :2984-2991
[7]   A microfabricated fluorescence-activated cell sorter [J].
Fu, AY ;
Spence, C ;
Scherer, A ;
Arnold, FH ;
Quake, SR .
NATURE BIOTECHNOLOGY, 1999, 17 (11) :1109-1111
[8]   An integrated microfabricated cell sorter [J].
Fu, AY ;
Chou, HP ;
Spence, C ;
Arnold, FH ;
Quake, SR .
ANALYTICAL CHEMISTRY, 2002, 74 (11) :2451-2457
[9]   Selective retention of viable cells in ultrasonic resonance field devices [J].
Gaida, T ;
DoblhoffDier, O ;
Strutzenberger, K ;
Katinger, H ;
Burger, W ;
Groschl, M ;
Handl, B ;
Benes, E .
BIOTECHNOLOGY PROGRESS, 1996, 12 (01) :73-76
[10]  
Gomez F.A., 2008, BIOL APPL MICROFLUID