Localization of Rac2 via the C terminus and aspartic acid 150 specifies superoxide generation, actin polarity and chemotaxis in neutrophils

被引：110

作者：

Filippi, MD

Harris, CE

Meller, J

Gu, Y

Zheng, Y

Williams, DA ^{[1
]}

机构：

[1] Univ Cincinnati, Coll Med, Div Expt Hematol, Cincinnati, OH 45229 USA

[2] Univ Cincinnati, Coll Med, Div Pediat Informat, Cincinnati Childrens Hosp Res Fdn, Cincinnati, OH 45229 USA

来源：

NATURE IMMUNOLOGY | 2004年 / 5卷 / 07期

关键词：

D O I：

10.1038/ni1081

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Despite having a high degree of sequence similarity, the Rho guanosine triphosphatases Rac1 and Rac2 regulate distinct functions in neutrophils. Here we demonstrate that the unique Rac2 localization and functions in neutrophils are regulated by two separate C-terminal motifs, the hypervariable domain and aspartic acid 150, one of which has not previously been linked to the function of Rho GTPases. In addition, we show an unexpected dependence of Rac1 localization on Rac2 activity in these same cells, demonstrating a degree of crosstalk between two closely related Rho GTPases. Thus, we have defined specific sequences in Rac that specify subcellular localization and determine the specificity of Rac2 in neutrophil chemotaxis and superoxide generation.

引用

页码：744 / 751

页数：8

共 37 条

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