Midinfrared surface-plasmon resonance: A novel biophysical tool for studying living cells

被引：53

作者：

Golosovsky, M. ^{[1
]}

Lirtsman, V. ^{[1
]}

Yashunsky, V. ^{[1
]}

Davidov, D. ^{[1
]}

Aroeti, B. ^{[2
]}

机构：

[1] Hebrew Univ Jerusalem, Racah Inst Phys, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Dept Cell & Anim Biol, Alexander Silberman Inst Life Sci, IL-91904 Jerusalem, Israel

来源：

JOURNAL OF APPLIED PHYSICS | 2009年 / 105卷 / 10期

关键词：

biological techniques; biomembranes; cellular biophysics; chemical analysis; chemical variables measurement; Fourier transform spectroscopy; infrared spectroscopy; surface plasmon resonance; FLUORESCENCE SPECTROSCOPY; SENSITIVE DETECTION; ULTRATHIN FILMS; BIOSENSORS; GLUCOSE; PROTEIN; MEMBRANES; SENSORS; BINDING; EVENTS;

D O I：

10.1063/1.3116143

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We discuss the surface-plasmon resonance (SPR) technique based on Fourier transform infrared (FTIR) spectrometry. We explore the potential of the infrared surface plasmon technique for biological studies in aqueous solutions and compare it with the conventional surface plasmon technique operating in the visible range. We demonstrate that the sensitivity of the SPR technique in the infrared range is not lower and in fact is even higher. We show several examples of applying FTIR-SPR for biological studies: (i) monitoring D-glucose concentration in solution and (ii) measuring D-glucose uptake by erythrocytes in suspension. We emphasize the advantages of infrared SPR for studying living cell cultures and show how this technique can be used for characterization of (i) cholesterol penetration into plasma membrane and (ii) transferrin-induced clathrin-mediated endocytosis.

引用

页数：11

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