Characterization of the mouse JAB1 cDNA and protein

被引：33
作者：
Bounpheng, MA
Melnikova, IN
Dodds, SG
Chen, HP
Copeland, NG
Gilbert, DJ
Jenkins, NA
Christy, BA [1 ]
机构：
[1] Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78245 USA
[2] Univ Texas, Hlth Sci Ctr, Inst Biotechnol, Dept Mol Med, San Antonio, TX 78245 USA
[3] NCI, Frederick Canc Res & Dev Ctr, Mammalian Genet Lab, ABL Basic Res Program, Frederick, MD 21702 USA
来源：
GENE | 2000年 / 242卷 / 1-2期
关键词：
chromosomal location; mRNA; proteasome; protein expression; subcellular location; ubiquitin;
D O I：
10.1016/S0378-1119(99)00525-9
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.
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收藏
页码：41 / 50
页数：10
相关论文
共 31 条
[1]   NUCLEAR LOCATION OF THE PUTATIVE TRANSFORMING PROTEIN OF AVIAN MYELOCYTOMATOSIS VIRUS [J].
ABRAMS, HD ;
ROHRSCHNEIDER, LR ;
EISENMAN, RN .
CELL, 1982, 29 (02) :427-439
[2]   JUVENILE SPERMATOGONIAL DEPLETION (JS']JSD) - A GENETIC-DEFECT OF GERM-CELL PROLIFERATION OF MALE-MICE [J].
BEAMER, WG ;
CUNLIFFEBEAMER, TL ;
SHULTZ, KL ;
LANGLEY, SH ;
RODERICK, TH .
BIOLOGY OF REPRODUCTION, 1988, 38 (04) :899-908
[3]   Covalent modification of the active site threonine of proteasomal beta subunits and the Escherichia coli homolog HslV by a new class of inhibitors [J].
Bogyo, M ;
McMaster, JS ;
Gaczynska, M ;
Tortorella, D ;
Goldberg, AL ;
Ploegh, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (13) :6629-6634
[4]   The COP9 complex, a novel multisubunit nuclear regulator involved in light control of a plant developmental switch [J].
Chamovitz, DA ;
Wei, N ;
Osterlund, MT ;
vonArnim, AG ;
Staub, JM ;
Matsui, M ;
Deng, XW .
CELL, 1996, 86 (01) :115-121
[5]  
Chang YC, 1998, CELL GROWTH DIFFER, V9, P79
[6]   The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor [J].
Chen, CF ;
Li, S ;
Chen, YM ;
Chen, PL ;
Sharp, ZD ;
Lee, WH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (51) :32863-32868
[7]   PARAMETERS AFFECTING THE INTENSITY OF WIND-DRIVEN RAIN ON THE FRONT FACE OF A BUILDING [J].
CHOI, ECC .
JOURNAL OF WIND ENGINEERING AND INDUSTRIAL AERODYNAMICS, 1994, 53 (1-2) :1-17
[8]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[9]   DIFFERENTIATION-INDUCED GENE-EXPRESSION IN 3T3-L1 PREADIPOCYTES - CCAAT ENHANCER BINDING-PROTEIN INTERACTS WITH AND ACTIVATES THE PROMOTERS OF 2 ADIPOCYTE-SPECIFIC GENES [J].
CHRISTY, RJ ;
YANG, VW ;
NTAMBI, JM ;
GEIMAN, DE ;
LANDSCHULZ, WH ;
FRIEDMAN, AD ;
NAKABEPPU, Y ;
KELLY, TJ ;
LANE, MD .
GENES & DEVELOPMENT, 1989, 3 (09) :1323-1335
[10]   A new group of conserved coactivators that increase the specificity of AP-1 transcription factors [J].
Claret, FX ;
Hibi, M ;
Dhut, S ;
Toda, T ;
Karin, M .
NATURE, 1996, 383 (6599) :453-457
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