Pit-1 gene expression in human lactotroph and somatotroph pituitary adenomas is correlated to D2 receptor gene expression

被引:16
作者
PellegriniBouiller, I
MorangeRamos, I
Barlier, A
Gunz, G
FigarellaBranger, D
CortetRudelli, C
Grisoli, F
Jaquet, P
Enjalbert, A
机构
[1] UNIV MEDITERRANEE, INST JEAN ROCHE, LAB INTERACT CELLULAIRES NEUROENDOCRINIENNES, UMR 9941 CNRS, F-13916 MARSEILLE 20, FRANCE
[2] SERV ENDOCRINOL, MARSEILLE, FRANCE
[3] SERV ENDOCRINOL, LILLE, FRANCE
[4] SERV NEUROCHIRURG, MARSEILLE, FRANCE
[5] NEUROPATHOL LAB, MARSEILLE, FRANCE
关键词
D O I
10.1210/jc.81.9.3390
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The expression of the pituitary-specific transcription factor Pit-1 gene was analyzed in a series of 30 human lactotroph and somatotroph pituitary tumors. Northern blot analysis failed to reveal any quantitative differences in Pit-1 gene expression between somatotroph and lactotroph tumors, and reverse transcription-PCR analysis showed similar patterns of Pit-1 isoforms expression in both populations of tumors. The expression of the D2 receptor gene was subsequently analyzed in the same adenomas. In the prolactinomas, which presented with a variable sensitivity to dopamine agonist treatment, the intensity of the D2 receptor transcripts (2.8 kilobases) was variable and was related to the sensitivity to the dopamine agonist treatment. Notably, the individual D2 receptor messenger ribonucleic acid mRNA) levels were highly correlated to the Pit-1 mRNA levels measured in the same tumors (r = 0.90; P < 0.0001). In the GH-secreting tumors, a significant expression of the D2 receptor gene was evidenced by Northern blot in all mixed somato-lactotroph adenomas and in some of the pure somatotroph adenomas; again, a positive correlation was found between D2 mRNA and Pit-1 mRNA levels (r = 0.68, P < 0.01). These results suggest the existence of mechanisms responsible for a coordinate control of Pit-1 and D2 receptor genes that remain to be determined.
引用
收藏
页码:3390 / 3396
页数:7
相关论文
共 37 条
  • [31] SCHAUFELE F, 1994, PITUITARY GLAND, P91
  • [32] THYROTROPIN-RELEASING-HORMONE REGULATION OF HUMAN TSHB EXPRESSION - ROLE OF A PITUITARY-SPECIFIC TRANSCRIPTION FACTOR (PIT-1/GHF-1) AND POTENTIAL INTERACTION WITH A THYROID HORMONE-INHIBITORY ELEMENT
    STEINFELDER, HJ
    HAUSER, P
    NAKAYAMA, Y
    RADOVICK, S
    MCCLASKEY, JH
    TAYLOR, T
    WEINTRAUB, BD
    WONDISFORD, FE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) : 3130 - 3134
  • [33] CRETINISM WITH COMBINED HORMONE DEFICIENCY CAUSED BY A MUTATION IN THE PIT1 GENE
    TATSUMI, K
    MIYAI, K
    NOTOMI, T
    KAIBE, K
    AMINO, N
    MIZUNO, Y
    KOHNO, H
    [J]. NATURE GENETICS, 1992, 1 (01) : 56 - 58
  • [34] DIFFERENTIAL SPLICING OF THE GHF1 PRIMARY TRANSCRIPT GIVES RISE TO 2 FUNCTIONALLY DISTINCT HOMEODOMAIN PROTEINS
    THEILL, LE
    HATTORI, K
    LAZZARO, D
    CASTRILLO, JL
    KARIN, M
    [J]. EMBO JOURNAL, 1992, 11 (06) : 2261 - 2269
  • [35] TRANSCRIPTION OF THE RAT DOPAMINE-D2-RECEPTOR GENE FROM 2 PROMOTERS
    VALDENAIRE, O
    VERNIER, P
    MAUS, M
    EDWARDS, JBDM
    MALLET, J
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 220 (02): : 577 - 584
  • [36] AN ALTERNATIVE PIT-1 RNA SPLICING PRODUCT REVEALS MODULAR BINDING AND NONMODULAR TRANSCRIPTIONAL ACTIVITIES OF THE POU-SPECIFIC DOMAIN
    VOSS, JW
    WILSON, L
    RHODES, SJ
    ROSENFELD, MG
    [J]. MOLECULAR ENDOCRINOLOGY, 1993, 7 (12) : 1551 - 1560
  • [37] ZAFFAR M, 1995, J CLIN ENDOCR METAB, V80, P3621