A two-photon fluorescent probe for biological Cu (II) and PPi detection in aqueous solution and in vivo

被引：66

作者：

Guo, Mengmeng ^{[1
]}

Dong, Pengyu ^{[2
]}

Feng, Yan ^{[1
]}

Xi, Xinguo ^{[2
]}

Shao, Rong ^{[2
]}

Tain, Xiaohe ^{[3
]}

Zhang, Buchang ^{[4
]}

Zhu, Manzhou ^{[1
]}

Meng, Xiangming ^{[1
]}

机构：

[1] Anhui Univ, Dept Chem, Hefei 230601, Peoples R China

[2] Yancheng Inst Technol, Jiangsu Collaborat Innovat Ctr Ecol Bldg Mat & En, Yancheng 224051, Peoples R China

[3] Anhui Univ, Sch Life Sci, Hefei 230601, Peoples R China

[4] Anhui Univ, Inst Hlth Sci, Hefei 230601, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2017年 / 90卷

基金：

中国国家自然科学基金;

关键词：

Two photon; Fluorescent probe; Pyrophosphate; Tissue imaging; In vivo imaging; LIVING CELLS; ALKALINE-PHOSPHATASE; LIVE TISSUES; METAL-IONS; PYROPHOSPHATE; RECOGNITION; ZN2+; CHEMOSENSORS; COMPLEXES; SENSOR;

D O I：

10.1016/j.bios.2016.11.069

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The first two-photon fluorescent probe (PC) for selectively detecting biological Cu (II) and pyrophosphate (PPi) has been developed based on 7-substituted coumarin in this study. The probe presented excellent selective two photon "on-off-on" detection signal for Cu(II) /PPi in aqueous solution. The two-photon detection ensemble (PCCu) can detect PPi released from DNA amplification after the polymerase chain reactions (PCR). The probe showed low cytotcodcity and good biocompatibility, and therefore can be applied for imaging Cu(II)/PPi in living cells under two-photon excitation. Furthermore, the ensemble probe (PCCu) was also used to image PPi in deep living rat tissues (similar to 100 mu m) and in 5-days old zebrafish.

引用

页码：276 / 282

页数：7

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