Design and fabrication of an electrochemical sensing platform based on a porous organic polymer for ultrasensitive ampicillin detection

A novel porous organic polymer (POP) was designed and prepared by the coupled polymerization of 1, 3, 6, 8-Tetraphenylpyrene and alpha, alpha'-dibromo-p-xylen. The as-synthesized POP is an extended pi-conjugation framework with high surface area, excellent stability and large pore cavity, thus bestowing the outstanding carrying capacity of aptamer strands through pi-pi stacking interaction to rationally fabricate a ultrasensitive electrochemical aptasensor. The biosensor exhibits the lowest limit of detection of 1.33 fg.mL(-1) (3.30 fM) for detecting trace ampicillin (AMP) by using electrochemical impedance spectroscopy. Furthermore, this biosensor can accurately monitor AMP in multiple real samples. This work provides the greatly potential applications of POPs in the fabrication of electrochemical biosensors for sensitively detecting antibiotics or other analytes.