The sequence of sequencers: The history of sequencing DNA

被引:652
作者
Heather, James M. [1 ]
Chain, Benjamin [1 ]
机构
[1] UCL, Div Infect & Immun, Cruciform Bldg,Gower St, London, England
基金
英国工程与自然科学研究理事会;
关键词
DNA; RNA; Sequencing; Sequencer; History; NUCLEOTIDE-SEQUENCE; SINGLE-MOLECULE; ENZYMATIC AMPLIFICATION; GENOME; GENERATION; POLYMERASE; OLIGONUCLEOTIDES; TECHNOLOGIES; INFORMATION; TERMINATORS;
D O I
10.1016/j.ygeno.2015.11.003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Determining the order of nucleic acid residues in biological samples is an integral component of a wide variety of research applications. Over the last fifty years large numbers of researchers have applied themselves to the production of techniques and technologies to facilitate this feat, sequencing DNA and RNA molecules. This time-scale has witnessed tremendous changes, moving from sequencing short oligonucleotides to millions of bases, from struggling towards the deduction of the coding sequence of a single gene to rapid and widely available whole genome sequencing. This article traverses those years, iterating through the different generations of sequencing technology, highlighting some of the key discoveries, researchers, and sequences along the way. (C) 2015 The Authors. Published by Elsevier Inc.
引用
收藏
页码:1 / 8
页数:8
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