Selection of appropriate reference genes in eggplant for quantitative gene expression studies under different experimental conditions

被引：20

作者：

Zhou, Xiaohui ^{[1
]}

Liu, Jun ^{[1
]}

Zhuang, Yong ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Peoples R China

来源：

SCIENTIA HORTICULTURAE | 2014年 / 176卷

关键词：

Quantitative real-time PCR; Reference gene; Gene expression; Eggplant (Solanum melongena L.); REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; RELIABLE REFERENCE GENES; RT-PCR; INTERNAL CONTROL; ACCURATE NORMALIZATION; HOUSEKEEPING GENES; SOLANUM-MELONGENA; VALIDATION; IDENTIFICATION;

D O I：

10.1016/j.scienta.2014.07.010

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Quantitative real-time polymerase chain reaction (RT-qPCR) has become one of the most widely used methods for gene expression analysis, and the evaluation of appropriate reference genes should be the first step to consider in experiments based on RT-qPCR. In this work, nine candidate reference genes, including 18sRNA, CYP, UBQ GAPDH, TUB, TUA, EF1, ACTIN and RPOB, were investigated for their expression stability in five different eggplant sample pools using three algorithms: geNorm, NormFinder and RefFinder. The samples were collected under different experimental conditions, including those from different plant tissues, hormone treatments, biotic and abiotic stresses. Our results demonstrated that the expression stability varied from different experimental conditions between reference genes. In general, GAPDH and 18sRNA had a good performance under most experimental conditions, whereas TUB and RPOB exhibited poor stability. This study should provide guidelines for the selection of reference genes for gene expression studies in eggplant. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：200 / 207

页数：8

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