Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair

被引：75
作者：
Fernandes, Sarah [1 ,2 ,3 ]
Chong, James J. H. [1 ,2 ,3 ,4 ,5 ]
Paige, Sharon L. [1 ,2 ,3 ]
Iwata, Mineo [8 ]
Torok-Storb, Beverly [8 ]
Keller, Gordon [9 ]
Reinecke, Hans [1 ,2 ,3 ]
Murry, Charles E. [1 ,2 ,3 ,6 ,7 ]
机构：
[1] Univ Washington, Ctr Cardiovasc Biol, Seattle, WA 98109 USA
[2] Univ Washington, Inst Stem Cell & Regenerat Med, Seattle, WA 98109 USA
[3] Univ Washington, Dept Pathol, Seattle, WA 98109 USA
[4] Univ Sydney, Sch Med, Sydney, NSW 2006, Australia
[5] Univ Sydney, Westmead Millennium Inst Med Res, Sydney, NSW 2145, Australia
[6] Univ Washington, Dept Bioengn, Seattle, WA 98109 USA
[7] Univ Washington, Dept Med Cardiol, Seattle, WA 98109 USA
[8] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA
[9] Ontario Canc Inst, McEwen Ctr Regenerat Med, Toronto, ON M5G 2M9, Canada
来源：
STEM CELL REPORTS | 2015年 / 5卷 / 05期
基金：
英国医学研究理事会;
关键词：
SKELETAL MYOBLAST TRANSPLANTATION; THERAPY; PERFORMANCE; INFARCTION;
D O I：
10.1016/j.stemcr.2015.09.011
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Cardiomyocytes derived from human embryonic stem cells (hESC-CMs) can improve the contractility of injured hearts. We hypothesized that mesodermal cardiovascular progenitors (hESC-CVPs), capable of generating vascular cells in addition to cardiomyocytes, would provide superior repair by contributing to multiple components of myocardium. We performed a head-to-head comparison of hESC-CMs and hESC-CVPs and compared these with the most commonly used clinical cell type, human bone marrow mononuclear cells (hBM-MNCs). In a nude rat model of myocardial infarction, hESC-CMs and hESC-CVPs generated comparable grafts. Both similarly improved systolic function and ventricular dilation. Furthermore, only rare human vessels formed from hESC-CVPs. hBM-MNCs attenuated ventricular dilation and enhanced host vascularization without engrafting long-term or improving contractility. Thus, hESC-CMs and CVPs show similar efficacy for cardiac repair, and both are more efficient than hBM-MNCs. However, hESC-CVPs do not form larger grafts or more significant numbers of human vessels in the infarcted heart.
引用
收藏
页码：753 / 762
页数：10
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