ZnO films formed by atomic layer deposition as an optical biosensor platform for the detection of Grapevine virus A-type proteins

被引：60
作者：
Tereshchenko, Alla [1 ]
Fedorenko, Viktoriia [1 ,2 ]
Smyntyna, Valentyn [1 ]
Konup, Igor [3 ]
Konup, Anastasiya [4 ]
Eriksson, Martin [5 ]
Yakimova, Rositsa [5 ]
Ramanavicius, Arunas [6 ]
Balme, Sebastien [2 ]
Bechelany, Mikhael [2 ]
机构：
[1] Odesa Natl II Mechnikov Univ, Expt Phys Dept, 42 Pastera, UA-65023 Odesa, Ukraine
[2] ENSCM UM CNRS UMR5635, Inst Europeen Membranes IEMM, Pl Eugene Bataillon, F-34095 Montpellier 5, France
[3] Odessa Natl II Mechnikov Univ, Dept Microbiol Virol & Biotechnol, 2 Shampanskiy Lane, UA-65000 Odesa, Ukraine
[4] Inst Viticulture & Wine Making, Natl Sci Ctr, 27 40 Let Pobedy Str, UA-65496 Odesa, Ukraine
[5] Linkoping Univ, Dept Phys Chem & Biol, S-58183 Linkoping, Sweden
[6] Vilnius Univ, Dept Phys Chem, Naugarduko Str 24, LT-03225 Vilnius, Lithuania
来源：
BIOSENSORS & BIOELECTRONICS | 2017年 / 92卷
关键词：
ZnO; Atomic layer deposition; Photoluminescence; Optical biosensor; Grapevine virus A-type; GOLD NANOPARTICLES; PHOTOLUMINESCENCE; IMMUNOSENSOR; IMMOBILIZATION; NANOSTRUCTURES; ENHANCEMENT; GLUCOSE; UV;
D O I：
10.1016/j.bios.2016.09.071
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Novel sensitive optical biosensor for determination of Grapevine virus A-type (GVA) proteins (GVA-antigens) has been designed. This biosensor was based on thin films of Zinc Oxide (ZnO) deposited by atomic layer deposition (ALD). The ZnO-based films have demonstrated favorable surface-structural properties for the direct immobilization of antibodies against OVA-antigens in order to form a biosensitive layer sensitive to OVA antigens. The immobilization was confirmed by intensity changes in the main near band emission (NBE) peak of ZnO and by the formation of intense photoluminescence band, discovered in the visible range around 425 nm, caused by the immobilized proteins. The GVA-antigen detection was performed by the evaluation of changes and behavior of a corresponding luminescence band. The sensitivity of as-formed label-free biosensor towards the OVA-antigens was determined in the range from 1 pg/ml to 10 ng/ml; in addition, the selectivity of biosensor was evaluated.
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收藏
页码：763 / 769
页数：7
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