Physical biology of GPCR signalling dynamics inferred from fluorescence spectroscopy and imaging

被引：10
作者：
Chavez-Abiega, Sergei [1 ,2 ]
Goedhart, Joachim [1 ]
Bruggeman, Frank Johannes [2 ]
机构：
[1] Univ Amsterdam, Swammerdam Inst Life Sci, Sect Mol Cytol, Sci Pk 904, NL-1098 XH Amsterdam, Netherlands
[2] Vrije Univ Amsterdam, Amsterdam Inst Mol Med & Syst, Sect Syst Bioinformat, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2019年 / 55卷
基金：
欧盟地平线“2020”;
关键词：
PROTEIN-COUPLED RECEPTOR; SINGLE-MOLECULE; BETA-ARRESTIN; MEDIATED PHOSPHORYLATION; BIOSENSORS REVEAL; ACTIVATION; EXPRESSION; SELECTIVITY; REGULATORS; DOPAMINE;
D O I：
10.1016/j.sbi.2019.05.007
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The physical biology of G protein-coupled receptor (GPCR) signalling can be inferred from imaging of single molecules and single living cells. In this opinion paper, we highlight recent developments in technologies to study GPCR signalling in vitro and in cyto. We start from mobility and localisation characteristics of single receptors in membranes. Subsequently, we discuss the kinetics of shifts in receptor conformation equilibrium due to allosteric binding events and G protein activation. We continue with recent insights into downstream signalling and the role of delayed negative feedback to suppress GPCR signalling. Finally, we discuss new strategies to reveal how the multiplex signalling responses of cells to ligand mixtures, mediated by their entire receptor arsenal, can be disentangled, using single-cell data.
引用
收藏
页码：204 / 211
页数：8
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