Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production

被引：47

作者：

Zhu, Li-Hua ^{[1
]}

Krens, Frans ^{[2
]}

Smith, Mark A. ^{[3
]}

Li, Xueyuan ^{[1
]}

Qi, Weicong ^{[2
]}

van Loo, Eibertus N. ^{[2
]}

Iven, Tim ^{[4
]}

Feussner, Ivo ^{[4
,5
,6
]}

Nazarenus, Tara J. ^{[7
,8
]}

Huai, Dongxin ^{[7
,8
,9
,10
]}

Taylor, David C. ^{[3
]}

Zhou, Xue-Rong ^{[11
,12
]}

Green, Allan G.

Shockey, Jay ^{[13
]}

Klasson, K. Thomas ^{[13
]}

Mullen, Robert T. ^{[14
]}

Huang, Bangquan ^{[15
]}

Dyer, John M. ^{[16
]}

Cahoon, Edgar B. ^{[7
,8
]}

机构：

[1] Swedish Univ Agr Sci, Dept Plant Breeding, Box 101, SE-23053 Alnarp, Sweden

[2] Wageningen UR Plant Breeding, POB 386, NL-6700 AJ Wageningen, Netherlands

[3] Natl Res Council Canada, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada

[4] Univ Gottingen, Albrecht von Haller Inst Plant Sci, Dept Plant Biochem, Justus von Liebig Weg 11, D-37077 Gottingen, Germany

[5] Univ Gottingen, Goettingen Ctr Mol Biosci GZMB, Dept Plant Biochem, Justus von Liebig Weg 11, D-37077 Gottingen, Germany

[6] Univ Gottingen, ICASEC, Dept Plant Biochem, Justus von Liebig Weg 11, D-37077 Gottingen, Germany

[7] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA

[8] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA

[9] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

[10] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China

[11] CSIRO Food & Nutr, Sydney, NSW, Australia

[12] CSIRO Agr, Canberra, ACT, Australia

[13] ARS, USDA, So Reg Res Ctr, Commod Utilizat Res Unit, New Orleans, LA 70124 USA

[14] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada

[15] Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China

[16] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

瑞典研究理事会; 美国国家科学基金会;

关键词：

ERUCIC-ACID; FATTY-ACID; BRASSICA-CARINATA; RAT-HEART; OIL; JOJOBA; TRANSFORMATION; SYSTEM; CDNA; WAX;

D O I：

10.1038/srep22181

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.

引用

页数：11

共 38 条

[1] Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata [J].

Babic, V ;

Datla, RS ;

Scoles, GJ ;

Keller, WA .

PLANT CELL REPORTS, 1998, 17 (03) :183-188

[2]

BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911

[3]

BLOMSTRAND R, 1975, ACTA MED SCAND, P51

[4] PHYSICAL-PROPERTIES OF SPERMACETI OIL IN SPERM WHALE [J].

CLARKE, MR .

JOURNAL OF THE MARINE BIOLOGICAL ASSOCIATION OF THE UNITED KINGDOM, 1978, 58 (01) :19-26

[5] Soybean Oil: Genetic Approaches for Modification of Functionality and Total Content [J].

Clemente, Tom E. ;

Cahoon, Edgar B. .

PLANT PHYSIOLOGY, 2009, 151 (03) :1030-1040

[6] A gateway cloning vector set for high-throughput functional analysis of genes in planta [J].

Curtis, MD ;

Grossniklaus, U .

PLANT PHYSIOLOGY, 2003, 133 (02) :462-469

[7] CHANGES IN FATTY-ACID COMPOSITION OF CARDIAC MITOCHONDRIAL PHOSPHOLIPIDS IN RATS FED RAPESEED OIL [J].

DEWAILLY, P ;

NOUVELOT, A ;

SEZILLE, G ;

FRUCHART, JC ;

JAILLARD, J .

LIPIDS, 1978, 13 (04) :301-304

[8] CHANGES IN RAT-HEART PHOSPHOLIPID COMPOSITION AFTER RAPESEED OIL FEEDING [J].

DEWAILLY, P ;

SEZILLE, G ;

NOUVELOT, A ;

FRUCHART, JC ;

JAILLARD, J .

LIPIDS, 1977, 12 (03) :301-306

[9] Plant triacylglycerols as feedstocks for the production of biofuels [J].

Durrett, Timothy P. ;

Benning, Christoph ;

Ohlrogge, John .

PLANT JOURNAL, 2008, 54 (04) :593-607

[10] Variation and inheritance of oil content and fatty acid composition in niger (Guizotia abyssinica) [J].

Geleta, Mulatu ;

Stymne, Sten ;

Bryngelsson, Tomas .

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2011, 24 (07) :995-1003

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