Characterization and biological activity of Pefloxacin-imidazole mixed ligands complexes

Solid complexes [M(PEF)(HIm)Cl-x(H2O)(m)]_nH(2)O, [M = Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cr(III)] and [Ag(PEF)(HIm)]center dot 2H(2)O; PEF = Pefloxacin, HIm = imidazole were prepared and characterized using elemental analyses, IR spectroscopy, conductance measurements, UV-Vis spectra, ESR of the copper complex, magnetic moments measurements and H-1 NMR spectra. The results have shown that Pefloxacin reacts as a bidentate ligand and is bound to the metal ions through the pyridone oxygen and one carboxylic oxygen, except for the silver(I) complex where the metal ion is chelated to the piperazinyl nitrogen. Thermal studies including TGA and DTA were also performed on the complexes in order to study their stabilities and activation energies, Delta E*, entropies Delta S*; enthalpies Delta H* and order of reactions n have been derived using Horowitz-Metzeger method calculations. The mixed ligands complexes were evaluated for their antibacterial activity against two bacterial species, namely Staphylococcus aureus (S. aureus), Escherichia coli (E. coli). Antifungal screening was studied against two species (Aspergillus flavus and Candida albicans). The complexes under investigation were found to possess better antibacterial effects than uncomplexed Pefloxacin as well as antifungal activity. (C) 2014 Elsevier B.V. All rights reserved.