Vanillin Improves and Prevents Trinitrobenzene Sulfonic Acid-Induced Colitis in Mice

被引:75
作者
Wu, Shih-Lu [2 ]
Chen, Jaw-Chyun [5 ]
Li, Chia-Cheng [3 ]
Lo, Hsin-Yi [3 ]
Ho, Tin-Yun [3 ,4 ]
Hsiang, Chien-Yun [1 ]
机构
[1] China Med Univ, Dept Microbiol, Taichung 40402, Taiwan
[2] China Med Univ, Dept Biochem, Taichung 40402, Taiwan
[3] China Med Univ, Grad Inst Chinese Med Sci, Taichung 40402, Taiwan
[4] China Med Univ Hosp, Dept Radiat Therapy & Oncol, Taichung, Taiwan
[5] Da Yeh Univ, Dept Med Bot & Healthcare, Changhua, Taiwan
关键词
INFLAMMATORY-BOWEL-DISEASE; NF-KAPPA-B; ESTABLISHED EXPERIMENTAL COLITIS; TUMOR-NECROSIS-FACTOR; CROHNS-DISEASE; TRANSCRIPTION FACTOR; ULCERATIVE-COLITIS; SIGNALING PATHWAY; CELLS; MODEL;
D O I
10.1124/jpet.109.152835
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Inflammatory bowel disease (IBD) is chronic inflammatory and relapsing disease of the gut. It has been known that activation of nuclear factor-kappa B (NF-kappa B) and production of proinflammatory cytokines play important roles in the pathogenesis of IBD. In this study, the effect of vanillin (4-hydroxy-3-methoxybenzaldehyde), a potent nuclear factor-kappa B (NF-kappa B) inhibitor, was evaluated in mice with trinitrobenzene sulfonic acid (TNBS)-induced colitis. Oral administration of vanillin improved macroscopic and histological features of TNBS-induced colitis in a dose-dependent manner. Vanillin not only prevented TNBS-induced colitis but also ameliorated the established colitis. By in vivo NF-kappa B bioluminescence imaging, electrophoretic mobility shift assay, and Western blot, we found that vanillin suppressed in vivo NF-kappa B activities through the inhibition of p65 translocation, inhibitor of nuclear factor-kappa B (I kappa B)-alpha phosphorylation, and I kappa B kinase activation. Furthermore, vanillin reduced the expressions of proinflammatory cytokines [interleukin (IL)-1 beta, IL-6, interferon-gamma, and tumor necrosis factor-alpha] and stimulated the expression of anti-inflammatory cytokine (IL-4) in colonic tissues. In conclusion, this work identified vanillin as an anti-inflammatory compound with the capacity to prevent and ameliorate TNBS-induced colitis. Due to its safety, vanillin could be a potent candidate for the treatment of IBD.
引用
收藏
页码:370 / 376
页数:7
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