Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain-swapped dimeric structural architecture

被引：17

作者：

Kaur, Gundeep ^{[1
]}

Dutta, Dipak ^{[2
]}

Thakur, Krishan Gopal ^{[1
]}

机构：

[1] Inst Microbial Technol, CSIR, GN Ramachandran Prot Ctr, Struct Biol Lab, Chandigarh 160036, India

[2] Inst Microbial Technol, CSIR, Mol Biochem Lab, Chandigarh 160036, India

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2014年 / 82卷 / 05期

关键词：

CarD; CarD/CdnL-like family; transcription regulation; RNA polymerase binding protein; Mycobacterium tuberculosis; X-ray crystallography; domain swapping; AUTOMATED STRUCTURE SOLUTION; DENSITY MODIFICATION; MYXOCOCCUS-XANTHUS; RBPA; RESISTANCE; REGULATOR; DOMAINS;

D O I：

10.1002/prot.24419

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mycobacterium tuberculosis (Mtb) CarD is an essential transcriptional regulator that binds RNA polymerase and plays an important role in reprogramming transcription machinery under diverse stress conditions. Here, we report the crystal structure of CarD at 2.3 angstrom resolution, that represents the first structural description of CarD/CdnL-Like family of proteins. CarD adopts an overall bi-lobed structural architecture where N-terminal domain resembles 'tudor-like' domain and C-terminal domain adopts a novel five helical fold that lacks the predicted leucine zipper structural motif. The structure reveals dimeric state of CarD resulting from beta-strand swapping between the N-terminal domains of each individual subunits. The structure provides crucial insights into the possible mode(s) of CarD/RNAP interactions. (C) 2013 Wiley Periodicals, Inc.

引用

页码：879 / 884

页数：6

共 33 条

[31] Interaction of CarD with RNA Polymerase Mediates Mycobacterium tuberculosis Viability, Rifampin Resistance, and Pathogenesis [J].