Short Cationic Peptide Derived from Archaea with Dual Antibacterial Properties and Anti-Infective Potential

被引：41

作者：

Candido, Elizabete S. ^{[1
,2
]}

Cardoso, Marlon H. ^{[1
,2
,4
]}

Chan, Lai Y. ^{[3
,4
]}

Torres, Marcelo D. T. ^{[5
,6
,7
,8
,9
,10
,13
,14
,15
]}

Oshiro, Karen G. N. ^{[2
,3
]}

Porto, William F. ^{[2
,11
]}

Ribeiro, Suzana M. ^{[2
]}

Haney, Evan F. ^{[12
]}

Hancock, Robert E. W. ^{[12
]}

Lu, Timothy K. ^{[5
,6
,7
,8
,9
]}

de la Fuente-Nunez, Cesar ^{[5
,6
,7
,8
,9
,13
,14
]}

Craik, David J. ^{[4
]}

Franco, Octavio L. ^{[1
,2
,3
]}

机构：

[1] Univ Catolica Brasilia, Ctr Anal Prote & Bioquim, Posgrad Ciencias Genom & Biotecnol, SGAN 916 Modulo B, BR-70790160 Brasilia, DF, Brazil

[2] Univ Catolica Dom Bosco, S Inova Biotech, Programa Posgrad Biotecnol, Ave Tamandare 6000, BR-79117900 Campo Grande, MG, Brazil

[3] Univ Brasilia, Fac Med, Programa Posgrad Patol Mol, Campus Darcy Ribeiro, BR-70910900 Brasilia, DF, Brazil

[4] Univ Queensland, Inst Mol Biosci, 306 Carmody Rd, Brisbane, Qld 4072, Australia

[5] MIT, MIT Synthet Biol Ctr, Synthet Biol Grp, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] MIT, Ctr Microbiome Informat & Therapeut, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[7] MIT, Elect Res Lab, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[8] MIT, Dept Elect Engn & Comp Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[9] Broad Inst MIT & Harvard, Cambridge, MA 02139 USA

[10] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Sao Paulo, Brazil

[11] Porto Reports, BR-70790160 Brasilia, DF, Brazil

[12] Univ British Columbia, Ctr Microbial Dis & Immun Res, Vancouver, BC V6T 1Z4, Canada

[13] Univ Penn, Perelman Sch Med, Dept Psychiat, Philadelphia, PA 19104 USA

[14] Univ Penn, Perelman Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA

[15] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA

来源：

ACS INFECTIOUS DISEASES | 2019年 / 5卷 / 07期

关键词：

antimicrobial peptide; biofilm; cutaneous infection; CD spectroscopy; ANTIMICROBIAL PEPTIDES; ANTIBIOFILM; STABILITY; BACTERIA;

D O I：

10.1021/acsinfecdis.9b00073

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Bacterial biofilms and associated infections represent one of the biggest challenges in the clinic, and as an alternative to counter bacterial infections, antimicrobial peptides have attracted great attention in the past decade. Here, ten short cationic antimicrobial peptides were generated through a sliding-window strategy on the basis of the 19 amino acid residue peptide, derived from a Pyrobaculum aerophilum ribosomal protein. PaDBS1R6F10 exhibited anti infective potential as it decreased the bacterial burden in murine Pseudomonas aeruginosa cutaneous infections by more than 1000-fold. Adverse cytotoxic and hemolytic effects were not detected against mammalian cells. The peptide demonstrated structural plasticity in terms of its secondary structure in the different environments tested. PaDBS1R6F10 represents a promising antimicrobial agent against bacteria infections, without harming human cells.

引用

页码：1081 / 1086

页数：11

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